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Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity

文献类型： 外文期刊

作者： Qin, Tao 1 ; Chen, Jin 2 ; Wang, Deyun 1 ; Hu, Yuanliang 1 ; Wang, Mi 1 ; Zhang, Jing 1 ; Nguyen, The Luong 1 ; Liu, Cui; 1 ;

作者机构： 1.Nanjing Agr Univ, Inst Tradit Chinese Vet Med, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing 210014, Jiangsu, Peoples R China

关键词： Chinese angelica polysaccharide;Selenylation modification;Lymphocyte proliferation

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN：

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收录情况： SCI

摘要： Chinese angelica polysaccharide (CAP) was extracted by water decoction and ethanol precipitation, purified through eliminating protein by Sevage method and column chromatography of Sephadex G-200, then selenizingly modified by nitric acid-sodium selenite method according to Lg(3~4) orthogonal design of three-factors, the usage amount of sodium selenite, reaction temperature and reaction time, at three level to obtain nine selenizing CAPs, sCAP1 -SCAP9. Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay taking the non-modified CAP as control. The results showed that selenylation modification could significantly enhance the immune-enhancing activity of CAP, sCAP2 presented best effect and the optimal modification conditions were 200 mg of sodium selenite for 500 mg of CAP, the reaction temperature of 70 GC and the reaction time of 6 h.

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