Characterization of physiological response and identification of associated genes under heat stress in rice seedlings
文献类型: 外文期刊
作者: Xue, Da-Wei 2 ; Jiang, Hua 1 ; Hu, Jiang 3 ; Zhang, Xiao-Qin 2 ; Guo, Long-Biao 3 ; Zeng, Da-Li 3 ; Dong, Guo-Jun 3 ; Sun 1 ;
作者机构: 1.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China
2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China
3.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
关键词: DNSH;Heat stress;Malondialdehyde;Microarray;QPCR;Rice (Oryza sativa L.)
期刊名称:PLANT PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:4.27; 五年影响因子:4.816 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Global warming, which is caused by greenhouse gas emissions, makes food crops more vulnerable to heat stress. Understanding the heat stress-related mechanisms in crops and classifying heat stress-related genes can increase our knowledge in heat-resistant molecular biology and propel developments in molecular design breeding, which can help rice cope with unfavorable temperatures. In this study, we carried out a physiological analysis of rice plants after heat stress. The results show a dramatic increase in malondialdehyde contents and SOD activities. We successfully isolated 11 heat-related rice genes with known function annotation through DNSH, which is an improved SSH method for screening long cDNA fragments. The reanalysis of microarray data from public database revealed that all these genes displayed various expression patterns after heat stress, drought, cold and salt. Quantitative real-time reverse transcription PCR was also performed to validate the expression of these genes after heat stress. The expressions in 10 genes were all significantly changed except for contig 77, which is a CBL-interacting protein kinase. Several reports have been published about the members of the same gene family.
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