Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining
文献类型： 外文期刊
作者： Xu, Dong 1 ; Gao, Zhengquan 2 ; Li, Feng 1 ; Fan, Xiao 1 ; Zhang, Xiaowen 1 ; Ye, Naihao 1 ; Mou, Shanli 1 ; Liang, Cheng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China
3.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China
4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin, Peoples R China
关键词： Biodiesel;BODIPY 505/515;Lipid determination;Nitrogen limitation;Tetraselmis subcordiformis
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
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收录情况： SCI
摘要： BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga Tetraselmis subcordiformis. A concentration of 0.28μgml~(-1) and staining for 6min was optimal. Lipid bodies stained with BODIPY505/515 had a characteristic green fluorescence. Their volumes were determined using the sphere volume formula. Lipid accumulation under different nitrogen concentrations was analyzed. With an increase in NaNO_3 concentration from 0 to 240mg L~(-1), the maximum algal concentration increased from 8.23±0.62 (×10~5 cells ml~(-1)) to 1.61±0.13 (×10~6 cellsml~(-1)), while the maximum volume of intracellular neutral lipid decreased from 9.78±1.77 μm~3cell~(-1) to 6.00±0.59μm~3cell~(-1). A comparison of the lipid contents measured by BODIPY 505/515 staining and the gravimetric method showed a positive correlation coefficient of R~2=0.93. BODIPY 505/515 staining is a promising method in lipid quantitation in T. subcordiformis.
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