中国热带农业科学院机构知识库

Detection of cucumber mosaic virus isolates from banana by one-step reverse transcription loop-mediated isothermal amplification

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文献类型：外文期刊

作者： Peng, Jun ¹ ; Shi, Minjing ⁴ ; Xia, Zihao ¹ ; Huang, Junsheng ³ ; Fan, Zaifeng ¹ ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr, Danzhou 571737, Hainan, Peoples R China

4.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Rubber Biol, Minist Agr, Danzhou 571737, Hainan, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （影响因子：2.574；五年影响因子：2.466 ）

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收录情况： SCI

摘要： Cucumber mosaic virus (CMV) is one of the most devastating threats to the banana industry. A single-tube, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of CMV-infected banana and plantain (Musa spp.). The reaction was performed in a single tube at 63 °C for 90 min using a real-time turbidimeter, with an improved closed-tube visual detection system in which fluorescent dye was added to the inside of the lid prior to amplification. This RT-LAMP assay is an alternative method for the rapid detection of CMV in banana plants and tissue culture materials.

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