Detection of cucumber mosaic virus isolates from banana by one-step reverse transcription loop-mediated isothermal amplification
文献类型： 外文期刊
作者： Peng, Jun 1 ; Shi, Minjing 4 ; Xia, Zihao 1 ; Huang, Junsheng 3 ; Fan, Zaifeng 1 ;
作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
2.China Agr Univ, Minist Agr, Key Lab Plant Pathol, Beijing 100193, Peoples R China
3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr, Danzhou 571737, Hainan, Peoples R China
4.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Rubber Biol, Minist Agr, Danzhou 571737, Hainan, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
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收录情况： SCI
摘要： Cucumber mosaic virus (CMV) is one of the most devastating threats to the banana industry. A single-tube, one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of CMV-infected banana and plantain (Musa spp.). The reaction was performed in a single tube at 63 °C for 90 min using a real-time turbidimeter, with an improved closed-tube visual detection system in which fluorescent dye was added to the inside of the lid prior to amplification. This RT-LAMP assay is an alternative method for the rapid detection of CMV in banana plants and tissue culture materials.
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