您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Electrochemical Behavior of Calcein and the Interaction Between Calcein and DNA

文献类型： 外文期刊

作者： Zhang, Xuzhi 1 ; Li, Meng 2 ; Cui, Yi 1 ; Zhao, Jun 1 ; Cui, Zhengguo 1 ; Li, Qiufen 1 ; Qu, Keming 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Shandong Prov Key Lab Fishery Resources & Ecol En, Qingdao 266071, Peoples R China

2.Dalian Ocean Univ, Sch Life Sci & Technol, Dalian 116023, Peoples R China

关键词： Calcein;Conductive carbon black paste electrode;DNA interaction;Electrochemical behavior;Voltammetry

期刊名称：ELECTROANALYSIS （ 影响因子：3.223； 五年影响因子：2.947 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The electrochemical behavior of calcein (CA) has been investigated by using a conductive carbon black paste electrode (CCBPE) as working electrode. It exhibits a single well-defined redox peak in phosphate buffered saline in the range of pH5.5-8.0, which attributes to the irreversible oxidation with 2 electrons and 2 protons participation. Under the optimized analytical conditions, the proposed linear sweep voltammetry (LSV) method allows the determination of CA in a linear concentration range of 0.64-9.60μM, with a limit of detection of 0.32μM. Further, the interaction between CA and DNA were studied by voltammetric and spectrometric methods. Both studies have shown that CA can bind to DNA by the intercalation binding mode. Under the present experimental condition, the binding constant β of CA and dsDNA is 1.10×10 ~7. Meanwhile, in the loop-mediated isothermal amplification (LAMP) reaction mixture there is obvious interaction between CA and dsDNA, resulting in a nonignorable decrease of the indicating sensitivity.

  • 相关文献

[1]Development and evaluation of reverse transcription loop-mediated isothermal amplification assay for the detection of the fathead minnow nidovirus. Zhang, Qingli,Zhang, Qingli,Standish, Isaac,Winters, Andrew D.,Faisal, Mohamed,Winters, Andrew D.,Faisal, Mohamed,Puzach, Corey,Ulferts, Rachel,Ziebuhr, John,Ziebuhr, John. 2014

[2]Simultaneous Detection of Diethylstilbestrol and Malachite Green Using Conductive Carbon Black Paste Electrode. Qu, Keming,Zhang, Xuzhi,Li, Meng,Cui, Zhengguo,Zhang, Yan,Chen, Bijuan,Ma, Shaosai,Lv, Zhilin,Kong, Qing,Li, Meng. 2012

[3]Recording the Reaction Process of Loop-Mediated Isothermal Amplification (LAMP) by Monitoring the Voltammetric Response of 2 '-Deoxyguanosine 5 '-Triphosphate. Zhang, Xuzhi,Qu, Keming,Li, Qiufen,Cui, Zhengguo,Zhao, Jun,Sun, Xuemei. 2011

[4]Voltammetric Behavior of Semicarbazide at Graphene Modified Electrode and Application to Detection. Zhang, Xuzhi,Qu, Keming,Gu, Xiaolei,Zhao, Changzhi. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台