您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and expression analysis of Dicer2 in black tiger shrimp (Penaeus monodon) responses to immune challenges

文献类型： 外文期刊

作者： Li, Xiaolan 1 ; Yang, Lishi 1 ; Jiang, Song 1 ; Fu, Mingjun 1 ; Huang, Jianhua 1 ; Jiang, Shigui 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

2.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Dicer2;Penaeus monodon;RNAi pathway;Bacteria;Antiviral innate immunity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Dicer is a key initiative protein of the RNA interference (RNAi) pathway that produces small interfering RNAs (siRNAs) or micro RNAs (miRNA), which then leads to RNA-directed gene regulation or viral immunity. In the present study, we identified and characterized a Dicer2 cDNA from black tiger shrimp Penaeus monodon (designated as PmDcr2). The full length cDNA of PmDcr2 contains a 5' untranslated region (UTR) of 109 bp, an open reading frame (ORF) of 4509 bp and a 3' UTR of 842 bp. The molecular weight (MW) of predicted PmDcr2 protein is 171.7 KDa with the theoretical isoelectric point of 6.23. PmDcr2 amino acid shared the highest similarity of 91.8% and 90.7% with Dicer2 of Litopenaeus vannamei and Marsupenaeus japonicas, respectively. Phylogenic analysis showed PmDcr2 was clustering with shrimp Dicer2, and closed to the insect group including Tribolium castaneum Dicer2. Real-time quantitative PCR showed that PmDcr2 was widely expressed in almost all examined tissues except muscle, with high expression in gill, hemocyte and lymph. The expression of PmDcr2 in hepatopancreas was up-regulated by Vibrio vulnificus and White Spot Syndrome Virus (WSSV), but not by Staphylococcus aureus. Furthermore, the viral nucleotide homologue dsRNA poly (I:C) and ssRNA R484 also remarkably induced PmDcr2 mRNA expression more efficient and stronger. These data reflect that PmDcr2 is not only response to the gram negative bacteria infection, but also specially to the viral infection in black tiger shrimp.

  • 相关文献

[1]Characterization of Argonaute2 gene from black tiger shrimp (Penaeus monodon) and its responses to immune challenges. Yang, Lishi,Li, Xiaolan,Jiang, Song,Qiu, Lihua,Zhou, Falin,Liu, Wenjing,Jiang, Shigui,Li, Xiaolan. 2014

[2]Isolation and characterization of homologous TRBP cDNA for RNA interference in Penaeus monodon. Yang, Lishi,Li, Xiaolan,Huang, Jianhua,Zhou, Falin,Su, Tianfeng,Jiang, Shigui,Li, Xiaolan. 2013

[3]南海西北部水域斑节对虾资源的调查研究. 钟振如,李辉权,张月平,陈敏. 1999

[4]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[5]Effect of guava leaves on growth and the non-specific immune response of Penaeus monodon. Yin, Xiao-Li,Li, Zhuo-Jia,Yang, Keng,Lin, Hei-Zhao,Guo, Zhi-Xun,Yin, Xiao-Li.

[6]Gene characteristics, immune and stress responses of PmPrx1 in black tiger shrimp (Penaeus monodon): Insights from exposure to pathogenic bacteria and toxic environmental stressors. Bu, Ruiqian,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Bu, Ruiqian,Bu, Ruiqian,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua,Bao, Weiyang.

[7]Characterization, expression and silencing by RNAi of p53 from Penaeus monodon. Dai, Wenting,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Dai, Wenting,Yang, Qibin.

[8]Effect of seven carbohydrate sources on juvenile Penaeus monodon growth performance, nutrient utilization efficiency and hepatopancreas enzyme activities of 6-phosphogluconate dehydrogenase, hexokinase and amylase. Niu, Jin,Lin, Hei-Zhao,Jiang, Shi-Gui,Chen, Xu,Wu, Kai-Chang,Tian, Li-Xia,Liu, Yong-Jian.

[9]Molecular cloning, characterization and expression analysis of thrombospondin gene from Penaeus monodon. Zhou, FaLin,Zheng, Liming,Zhang, Dianchang,Huang, JianHua,Qiu, Lihua,Yang, QiBin,Jiang, ShiGui,Zhou, FaLin,Zheng, Liming.

[10]Estimates of heritability and genetic correlations for growth-related traits in the tiger prawn Penaeus monodon. Sun, Miao Miao,Huang, Jian Hua,Jiang, Shi Gui,Yang, Qi Bin,Zhou, Fa Lin,Zhu, Cai Yan,Yang, Li Shi,Su, Tian Feng.

[11]Cloning, characterization, expression analysis and RNAi of Retinoblastoma-like gene from black tiger shrimp (Penaeus monodon). Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Xie, Bobo,Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua.

[12]Molecular cloning and mRNA expression of M-phase phosphoprotein 6 gene in black tiger shrimp (Penaeus monodon). Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang,Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang.

[13]Observations of reproductive development and maturation of male Penaeus monodon reared in tidal and earthen ponds. Jiang, Shi-Gui,Huang, Jian-Hua,Zhou, Fa-Lin,Chen, Xu,Yang, Qj-Bing,Wen, Wei-Geng,Ma, Zi-Ming.

[14]Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activity. Yang, Lishi,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan,Qiu, Lihua,Huang, Jianhua,Jiang, Shigui,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan. 2013

[15]The response of glutathione peroxidase 1 and glutathione peroxidase 7 under different oxidative stresses in black tiger shrimp, Penaeus monodon. Liu, Wei,Zhao, Chao,Wang, Pengfei,Qiu, Lihua,Liu, Wei,Lin, Heizhao,Qiu, Lihua,Wang, Shu. 2018

[16]Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon. Zhao, C.,Fu, M. J.,Qiu, L. H.,Zhao, C.,Fu, M. J.,Qiu, L. H.,Qiu, L. H.. 2016

[17]Morphometric analysis of four different populations of Penaeus monodon (Crustacea, Decapoda, Penaeidae). Sun, Miao Miao,Huang, Jian Hua,Jiang, Shi Gui,Yang, Qi Bin,Zhou, Fa Lin,Zhu, Cai Yan,Yang, Li Shi,Su, Tian Feng. 2013

[18]Characterization and expression analysis of a cyclin B gene from black tiger shrimp (Penaeus monodon). Zhao, C.,Fu, M. J.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Zhao, C.,Fu, M. J.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Yang, Q. B.. 2015

[19]Dietary values of astaxanthin and canthaxanthin in Penaeus monodon in the presence and absence of cholesterol supplementation: effect on growth, nutrient digestibility and tissue carotenoid composition. Niu, Jin,Li, Chun-Hou,Chen, Xu,Huang, Zhong,Lin, Hei-Zhao,Liu, Yong-Jian,Tian, Li-Xia. 2012

[20]Molecular Analysis of a Selenoprotein M Gene from Penaeus monodon and its Expression at Different Ovarian Stages of Development. Zhou, FaLin,Qiu, Lihua,Huang, JianHua,Yang, QiBin,Fu, Mingjun,Jiang, ShiGui,Zhou, FaLin,Qiu, Lihua,Huang, JianHua,Yang, QiBin,Fu, Mingjun,Jiang, ShiGui. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台