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Real-time PCR quantification of latent infection of wheat powdery mildew in the field

文献类型： 外文期刊

作者： Zheng, Yaming 1 ; Luo, Yong 2 ; Zhou, Yilin 1 ; Zeng, Xiaowei 1 ; Duan, Xiayu 1 ; Cao, Xueren 1 ; Song, Yuli 3 ; Wang, Ba 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China

3.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China

4.Northwest A&F Univ, Dept Plant Pathol, Yangling 712100, Peoples R China

关键词： Blumeria graminis f. sp. tritici;Real-time PCR;Disease monitoring;Molecular epidemiology

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN：

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收录情况： SCI

摘要： Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a destructive wheat disease worldwide. The key issue for the disease forecast is to timely and accurately estimate and quantify the latent infection levels in volunteer seedlings where the pathogen over-winters or over-summers to serve as sources of initial inoculum of epidemics. To improve the conventional method, a real-time PCR assay had been established in this study to quantify latent infection level of wheat leaves. Artificially and naturally infected leaves in wheat fields at different geographical locations in China were collected and processed to determine the latent infection levels. Linear relationships between the molecular disease index (MDX) and the observed disease index (DX) were obtained from artificial inoculation experiments. Field experiments showed that the spatial distribution patterns of MDX matched well with those of DX in the most cases. This study demonstrated that the real-time PCR assay was a useful tool to rapidly and accurately quantify the latent infection levels of wheat powdery mildew and to efficiently estimate the initial inoculum potentials of epidemics in the fields.

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