文献类型： 外文期刊

作者： Gao Quanxin ¹ ; Qi Lili ¹ ; Wu Tianxing ¹ ; Xia Tingting ¹ ; Wang Jinbo ¹ ;

作者机构： 1.Zhejiang Univ, Ningbo Inst Technol, Key Lab Mol Design & Nutr Engn, Ningbo, Zhejiang, Peoples R China

2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr, Key Lab Marine & Estuarine Fisheries, Shanghai, Peoples R China

关键词： Clostridium butyricum;HT-29 cells;NF-kB/IkB;toll-like receptors

期刊名称：ANIMAL CELLS AND SYSTEMS （ 影响因子：1.815； 五年影响因子：1.336 ）

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收录情况： SCI

摘要： Oral administration of Clostridium butyricum as probiotics is increasingly gaining importance in the treatment of intestinal inflammations and improvement of animal performance. The mechanisms of host cell receptor recognition of C. butyricum and downstream immune signaling pathways leading to this beneficial event, however, remain unclear. In this study, C. butyricum was investigated for its capability to influence the innate immune response of HT-29 cells to explore its mechanism of action. Our data showed that C. butyricum was able to stimulate toll-like receptor 2 (TLR2) production at mRNA level; however, TLR4, TLR5, TLR9, and myeloid differentiation primary response protein 88 (MyD88) transcription levels remain unaltered. The nuclear factor B (NF-B), interleukin-8 (IL-8), and tumor necrosis factor alpha (TNF-) levels in response to C. butyricum were significantly increased, indicating that HT-29 cells were sensitised by C. butyricum. It is the first time that our findings have showed the specific signaling pathways in HT-29 cells stimulated by C. butyricum, which, at least in part, can help to explain the beneficial properties of C. butyricum.