A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species
文献类型： 外文期刊
作者： An, Zewei 1 ; Li, Yachao 1 ; Xie, Lili 1 ; Zhai, Qilin 1 ; Huang, Huasun 2 ;
作者机构： 1.Hainan Univ, Coll Agr, Haikou 570228, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, State Ctr Rubber Breeding, Danzhou 571737, Hainan, Peoples R China
3.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Hainan, Peoples R China
关键词： low molecular weight RNA;microRNA;polyethylene glycol;plant
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
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收录情况： SCI
摘要： We have developed a time- and cost-effective method for isolating low molecular weight (LMW) RNA from plants. In our protocol, the isolation procedure can be completed within 3h. Polyethylene glycol (PEG) and absolute ethanol are used to isolate LMW RNA, and the LMW RNA yields were >80 μg/g of fresh-weight tissues for several of the plant species tested.
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