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Anatomical and physiological differences and differentially expressed genes between the green and yellow leaf tissue in a variegated chrysanthemum variety

文献类型： 外文期刊

作者： Chang, Qingshan 1 ; Chen, Sumei 1 ; Chen, Yu 1 ; Deng, Yanming 1 ; Chen, Fadi 1 ; Zhang, Fei 1 ; Wang, Shuwei 4 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

2.Henan Univ Sci & Technol, Coll Forestry, Luoyang 471003, Henan, Peoples R China

3.Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China

4.Shanghai Oe Biotech Co Ltd, ZhangJiang Area, Shanghai 201210, Peoples R China

关键词： Chrysanthemum;Real-time quantitative RT-PCR;Semi-quantitative RT-PCR;Suppression subtractive hybridization

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

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收录情况： SCI

摘要： The leaves of the chrysanthemum variety 'NAU04-1-31-1' are variegated with distinct green and yellow sectors. The chlorophyll content of the yellow leaf tissue is less than that in the green one. The chloroplasts in the yellow leaf tissue were vacuolated, lacked thylakoid membrane structure and contained clusters of plastoglobuli with few or no starch grains. The yellow leaf tissue was more sensitive to photo-inhibition than the green leaf tissue. Suppression subtractive hybridization (SSHs) libraries were constructed to identify genes which were differentially transcribed in the two tissue types. The sequencing of 339 SSH clones identified 150 unigenes (93 singletons and 57 contigs), of which 85 were differentially transcribed in the green leaf tissue and 65 in the yellow leaf tissue. Unigenes associated with photosynthesis were particularly frequent, and many of these genes were up-regulated in the yellow leaf tissue. Both CmChlH which encodes the large subunit of Mg-chelatase and CmFtsH (ATP-dependent metalloprotease) were up-regulated in the yellow leaf tissue, and their transcription was regulated by light.

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