文献类型： 外文期刊

作者： Fu, Mei-ying ¹ ; Chen, Mian-cai ¹ ; Xiao, Tong-bin ¹ ; Wang, Hui-fang ¹ ;

作者机构： 1.Hainan Acad Agr Sci, Hainan Key Lab Control Plant Dis & Insect Pests, Inst Environm & Plant Protect, Haikou 571100, Peoples R China

关键词： Hainan;nematode identification;mtDNA-PCR-RFLP;root-knot nematodes;southern herbs

期刊名称：RUSSIAN JOURNAL OF NEMATOLOGY （ 影响因子：0.286； 五年影响因子：0.464 ）

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收录情况： SCI

摘要： Various kinds of southern herbs have been planted in Hainan island because of the suitable climatic conditions. However, root-knot nematodes have become a serious problem to cultivation of southern herbs. Thirty Meloidogyne populations on southern herbs from Hainan island were characterised in terms of perineal pattern and esterase phenotype, and Hinjl and EcoRl was used to double digest the amplified products from the mitochondrial DNA (mtDNA) region between the COII and IrRNA genes. Perineal patterning and esterase phenotyping revealed that 17 of the populations were M. incognita, five were M. javanica, three were M. arenaria, four were M. enterolobii and one was M. hispanica. In order for rapid and reliable identification of Meloidogyne species,we used the primers C2F3 and 1108 to amplify the intergenic region between COII and IrRNA genes of mtDNA, and Hinfi and EcoRl were used to double digest the amplified products. The results showed that five Meloidogyne species can be distinguished. It isconcluded that mtDNA-PCR-RFLP was a rapid and reliable approach for molecular identification of common Meloidogyne species.