您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Over-expression of GmMYB39 leads to an inhibition of the isoflavonoid biosynthesis in soybean (Glycine max. L)

文献类型： 外文期刊

作者： Liu, Xiaoqing 1 ; Yuan, Lingling; Xu, Ling 1 ; Xu, Zhaolong 1 ; Huang, Yihong 1 ; He, Xiaolan 1 ; Ma, Hongxiang 1 ; Yi 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Bioresources Saline Soils, Prov Key Lab Agrobiol, Inst Biotechnol, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Bioresources Saline

关键词： Isoflavonoids synthesis;MYB;Soybean;Transcriptional factors

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this paper, we isolated and characterized a gene encoding the soybean MYB transcription factor, GmMYB39 (Accession No: XM_003538605). Analysis of the deduced amino acid sequence revealed that GmMYB39 contained N-terminal R2R3 repeats that corresponded to the DNA-binding domain of plant MYB-type proteins, which were highly conserved among the R2R3-MYB proteins. The detailed expression pattern of GmMYB39 in various tissues of soybean was investigated by quantitative RT-PCR. The transcript level was found to be higher in flowers than in other examined organs. In contrast, the GmMYB39 expression level was relatively weak in the pod. The GmMYB39-GFP fusion protein was found to localize in the nucleus of Arabidopsis mesophyll protoplasts. Over-expression of GmMYB39 in hairy roots resulted in a significant reduction of the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Whereas, the IFS transcript level was slightly but not significantly increased, and no significant change in CHI expression was observed between over-expression and control roots. The repressing effect on expression of CHS was further supported by the results from co-transfection assays of CHS promoter (reporter) and GmMYB39 (effector) in soybean. Compared to the control (only with the reporter construct), a significant decrease in the GUS activity was observed in soybean hairy roots with both effector and reporter constructs. Furthermore, an ultimate decrease in isoflavonoids contents coincided with the decrease in the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Overall, the results suggest that GmMYB39 plays an inhibiting role in regulating the isoflavonoid biosynthesis in soybean.

  • 相关文献

[1]影响葡萄果皮颜色的MYB类转录因子的研究进展. 江楠,张雷,李春霞,任晨琛,周步海. 2014

[2]利用酵母双杂交系统筛选花生AhMYB44互作蛋白. 刘永惠,沈一,沈悦,陈志德. 2020

[3]森林草莓MYB家族鉴定及生物信息学分析. 袁华招,于红梅,夏瑾,庞夫花,陈晓东,赵密珍. 2019

[4]转TaPIMP1基因小麦耐旱性的分析（英文）. 姚金保,杨学明,张增艳,张鹏,周小青,马鸿翔. 2014

[5]转TaPIMP1基因小麦耐旱性的分析（英文）. 周淼平,姚金保,杨学明,张增艳,张鹏,周小青,马鸿翔. 2014

[6]转MYB基因小麦耐旱性的初步分析. 周淼平,周小青,姚金保,张增艳,张鹏,杨学明,马鸿翔. 2013

[7]Validation and high-resolution mapping of a major quantitative trait locus for alkaline salt tolerance in soybean using residual heterozygous line. Tuyen, D. D.,Zhang, H. M.,Xu, D. H.,Tuyen, D. D.,Zhang, H. M..

[8]Heterologous expression of GmSIP1;3 from soybean in tobacco showed and growth retardation and tolerance to hydrogen peroxide. Zhang, Dayong,Huang, Yihong,Wan, Qun,Xu, Zhaolong,Shao, Hongbo,Shao, Hongbo,Kumar, Manoj,Pandey, Girdhar K..

[9]Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library. Xia, Zhengjun,Wu, Hongyan,Xia, Zhengjun,Watanabe, Satoshi,Harada, Kyuya.

[10]Identification of novel loci for salt stress at the seed germination stage in soybean. Kan, Guizhen,Li, Yakai,Hu, Zhenbin,Zhang, Wei,He, Xiaohong,Yu, Deyue,Ning, Lihua.

[11]Genome-wide analysis of terpene synthases in soybean: Functional characterization of GmTPS3. Huang, Fang,Wang, Xia,Wang, Jiao,Yu, Deyue,Liu, Jianyu,Zhang, Man,Zheng, Rui.

[12]The Soybean Basic Helix-Loop-Helix Transcription Factor ORG3-Like Enhances Cadmium Tolerance via Increased Iron and Reduced Cadmium Uptake and Transport from Roots to Shoots. Xu, Zhaolong,Liu, Xiaoqing,He, Xiaolan,Xu, Ling,Huang, Yihong,Shao, Hongbo,Zhang, Dayong,Shao, Hongbo,Tang, Boping,Ma, Hongxiang. 2017

[13]Over-expression of GmWRKY111 Enhances NaCl Tolerance of Salt-Sensitive Genotype of Glycine max. Xu, Zhao Long,Ma, Hong Xiang,Xu, Zhao Long,Yi, JinXin,Xu, Ling,Liu, Xiao Qing,He, XiaoLan,Huang, Yi Hong,Ma, Hong Xiang,Zhang, Da Yong,Ali, Zulfiqar,Karim, Ihsan,Rasul, Maria,Ali, Zulfiqar,Karim, Ihsan,Rasul, Maria. 2014

[14]Molecular identification of genes controlling flowering time, maturity, and photoperiod response in soybean. Xia, Zhengjun,Zhai, Hong,Liu, Baohui,Kong, Fanjiang,Yuan, Xiaohui,Wu, Hongyan,Xia, Zhengjun,Cober, Elroy R.,Harada, Kyuya. 2012

[15]Whole-genome identification and expression analysis of K+ efflux antiporter (KEA) and Na+/H+ antiporter (NHX) families under abiotic stress in soybean. Chen Hua-tao,Chen Xin,Wu Bing-yue,Yuan Xing-xing,Zhang Hong-mei,Cui Xiao-yan,Liu Xiao-qing. 2015

[16]A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress. Zhang, Dayong,He, Xiaolan,Xu, Zhaolong,Xu, Ling,Wei, Peipei,Huang, Yihong,Brestic, Marian,Ma, Hongxiang,Shao, Hongbo,Tong, Jinfeng,Brestic, Marian,Shao, Hongbo. 2016

[17]Soybean C2H2-Type Zinc Finger Protein GmZFP3 with Conserved QALGGH Motif Negatively Regulates Drought Responses in Transgenic Arabidopsis. Zhang, Dayong,Xu, Zhaolong,Wei, Peipei,Xu, Ling,Wan, Qun,Huang, Yihong,He, Xiaolan,Shao, Hongbo,Ma, Hongxiang,Tong, Jinfeng,Yang, Jiayin,Shao, Hongbo. 2016

[18]GmHKT1;4, a novel soybean gene regulating Na+/K+ ratio in roots enhances salt tolerance in transgenic plants. Chen, Huatao,Chen, Xin,Gu, Heping,Wu, Bingyue,Zhang, Hongmei,Yuan, Xingxing,Cui, Xiaoyan. 2014

[19]Purification and characterization of beta-glucosidase from newly isolated Aspergillus sp MT-0204. Qi, Bin,Liu, Xianjin,Qi, Bin,Wang, Limei. 2009

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台