文献类型： 外文期刊
作者： Zhang, Lan 1 ; Luo, Yanzhong 1 ; Zhu, Yongxing 2 ; Zhang, Liang 1 ; Zhang, Wei 1 ; Chen, Rumei 1 ; Xu, Miaoyun 1 ; Fan, Y 1 ;
作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Biotechnol Res Inst, Beijing 100081, Peoples R China
2.Ningxia
关键词： Glycine Max;Maize;Vitamin E;Tocopherol;gamma-Tocopherol methyltransferase (gamma-TMT) alpha-Tocopherol;GmTMT2a
期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）
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收录情况： SCI
摘要： Tocochromanol, or vitamin E, plays a crucial role in human and animal nutrition and is synthesized only by photosynthetic organisms. gamma-Tocopherol methyltransferase (gamma-TMT), one of the key enzymes in the tocopherol biosynthetic pathway in plants, converts gamma, delta-tocopherols into alpha-, beta-tocopherols. Tocopherol content was investigated in 15 soybean cultivars and GmTMT2 was isolated from five varieties based on tocopherol content. GmTMT2a was expressed in E. coli and the purified protein effectively converted gamma-tocopherol into alpha-tocopherol in vitro. Overexpression of GmTMT2a enhanced alpha-tocopherol content 4-6-fold in transgenic Arabidopsis, and alpha-tocopherol content increased 3-4.5-fold in transgenic maize seed, which correlated with the accumulation of GmTMT2a. Transgenic corn that is alpha-tocopherol-rich may be beneficial for animal health and growth.
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