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A one-step RT-PCR assay to detect and discriminate porcine reproductive and respiratory syndrome viruses in clinical specimens

文献类型： 外文期刊

作者： Yang, Keli 1 ; Li, Yanhe 2 ; Duan, Zhengying 1 ; Guo, Rui 1 ; Liu, Zewen 1 ; Zhou, Danna 1 ; Yuan, Fangyan 1 ; Tian, Yong 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Peoples R China

2.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China

关键词： Highly pathogenic;One-step RT-PCR;Porcine reproductive and respiratory syndrome virus;Viral discrimination

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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收录情况： SCI

摘要： Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) have led to large economic losses and, subsequently, have drawn great attention to its diagnosis and prevention. To facilitate rapid discrimination of HP-PRRSV from classical PRRSV (C-PRRSV), we developed a one-step RT-PCR assay. Primer specificities were evaluated with RNA extracted from 8 viral strains and our results revealed that the primers had a high specificity for PRRSV. The assay sensitivity was 25. copies/μL for both HP-PRRSV and C-PRRSV. A total of 929 serum samples were identified, of which 20.45% were HP-PRRSV-positive and 1.51% were C-PRRSV-positive, which was completely consistent with that of immunochromatochemistry and sequencing method. The proposed assay can detect the virus 2. days prior the onset of symptoms and it can be performed in 2. h, thereby providing a rapid method to discriminate HP-PRRSV from C-PRRSV for the identification and prevention of PRRSV infections.

  • 相关文献

[1]A Multiplex RT-PCR Assay to Detect and Discriminate Porcine Reproductive and Respiratory Syndrome Viruses in Clinical Specimens. Yang, Keli,Tian, Yongxiang,Zhou, Danna,Duan, Zhengying,Guo, Rui,Liu, Zewen,Yuan, Fangyan,Liu, Wei. 2017

[2]A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification. Kang, Kang,Luo, Jun,Kang, Kang,Zhong, Jiasheng,Zhang, Limin,Gou, Deming,Yang, Keli,Tian, Yongxiang,Zhai, Jianxin,Zhang, Li,Song, Changxu,Gou, Christine Yuan. 2014

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