您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Method for Molecular Analysis of Catalase Gene Diversity in Seawater

文献类型： 外文期刊

作者： Wang, Wei 1 ; Ji, Xiaofeng 1 ; Yuan, Cui 1 ; Dai, Fangqun 1 ; Zhu, Jiancheng 1 ; Sun, Mi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Yellow Sea Fisheries Res Inst, Marine Prod Resource & Enzyme Engn Lab, Qingdao 266071, Shandong, Peoples R China

关键词： Hydrogen peroxidase;Marine bacteria;PCR-RFLP method;Surface seawater

期刊名称：INDIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.461； 五年影响因子：2.259 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Catalase plays an important role in the metabolism of marine bacteria and has potential impact on the marine environment. Four PCR primers were designed to amplify the catalase gene fragments in marine bacteria by applying metagenomic DNA from Yellow Sea surface water as the template. Of the four reproducible target PCR products, the longest one with 900 bp were chosen for catalase gene library construction by the T-vector and the white Escherichia coli colonies in the library was screened through restriction-digesting the reamplified insert fragments by the selected restriction endonuclease MboI, and then the bands of the resulting products were displayed in the agarose gel by electrophoresis. The unique restriction fragment length polymorphism (RFLP) pattern was selected and the corresponding catalase gene fragments were sequenced, which verified that every unique RFLP pattern represented one type of catalase. This PCR-RFLP method above was established to investigate the bacterial catalase diversity in seawater.

  • 相关文献

[1]Diversity of bacterioplankton in the surface seawaters of Drake Passage near the Chinese Antarctic station. Xing, Mengxin,Li, Zhao,Wang, Wei,Sun, Mi. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台