浙江省农业科学院机构知识库

Nucleo-cytoplasmic shuttling of VPg encoded by Wheat yellow mosaic virus requires association with the coat protein

收藏
分享
全文链接

文献类型：外文期刊

作者： Sun, Liying ¹ ; Jing, Bian ² ; Andika, Ida Bagus ¹ ; Hu, Yingchun ³ ; Sun, Bingjian ⁴ ; Xiang, Rong ² ; Kondo, Hideki; ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Minist Agr,Key Lab Biotechnol Plant Protect, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China

3.Peking Univ, Coll Life Sci, Minist Educ Cell Proliferat & Different, Key Lab, Beijing 100871, Peoples R China

4.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Henan Province, Peoples R China

5.Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan

关键词： Nucleo-cytoplasmic shuttling;requires association;protein

期刊名称：JOURNAL OF GENERAL VIROLOGY （影响因子：3.891；五年影响因子：3.719 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： VPg (virus protein, genome-linked) is a multifunctional protein that plays important roles in viral multiplication in the cytoplasm. However, a number of VPgs encoded by plant viruses target the nucleus and this appears to be biologically significant. These VPgs may therefore be translocated between nuclear and cytoplasmic compartments during virus infection, but such nucleo-cytoplasmic transport has not been demonstrated. We report that VPg encoded by Wheat yellow mosaic virus (WYMV, genus Bymovirus, family Potyviridae) accumulated in both the nucleus and cytoplasm of infected cells, but localized exclusively in the nucleus when expressed alone in plants. Computational analyses predicted the presence of a nuclear localization signal (NLS) and a nuclear export signal (NES) in WYMV VPg. Mutational analyses showed that both the N-terminal and the NLS domains of VPg contribute to the efficiency of nuclear targeting. In vitro and in planta assays indicated that VPg interacts with WYMV coat protein (CP) and proteinase 1 (P1) proteins. Observation of VPg fused to a fluorescent protein and subcellular fractionation experiments showed that VPg was translocated to the cytoplasm when co-expressed with CP, but not with P1. Mutations in the NES domain or treatment with leptomycin B prevented VPg translocation to the cytoplasm when co-expressed with CP. Our results suggest that association with CP facilitates the nuclear export of VPg during WYMV infection.

相关文献

[1]Protein fractions in barley grains as affected by some agronomic factors and their relationships to malt quality. Wang, J. M.,Chen, J. X.,Dai, F.,Wu, F. B.,Yang, J. M.,Zhang, G. P.. 2007

[2]Genotypic and environmental variation in phytic acid content and its relation to protein content and malt quality in barley. Dai, Fel,Wang, Junmei,Zhang, Saihua,Xu, Zhenzhen,Zhang, Guoping. 2007

[3]Separation of Recombinant Geranylgeranyl Diphosphate Synthase of Deinococcus radiodurans from Expressed Strain Cell Homogenate by Immobilized Metal Affinity Chromatography on a Characterized Monolithic Cryogel Column. Shen Shaochuan,Wang Liangyan,Li Mingfeng,Liu Chengzhi,Tian Bing,Hua Yuejin,Shen Shaochuan,Yun Junxian,Sun Zongtao. 2013

[4]Domains of the cucumber mosaic virus 2b silencing suppressor protein affecting inhibition of salicylic acid-induced resistance and priming of salicylic acid accumulation during infection. Zhou, Tao,Murphy, Alex M.,Lewsey, Mathew G.,Westwood, Jack H.,Zhang, Heng-Mu,Gonzalez, Inmaculada,Carr, John P.,Zhou, Tao,Zhang, Heng-Mu,Gonzalez, Inmaculada,Canto, Tomas.

[5]Recovery of infectious rabbit hemorrhagic disease virus from rabbits after direct inoculation with in vitro-transcribed RNA. Liu, GQ,Zhang, YY,Ni, Z,Yun, T,Sheng, ZT,Liang, HL,Hua, JG,Li, SM,Du, QY,Chen, JP.

[6]Improvement of recombinant baculovirus infection efficiency to express manganese superoxide dismutase in silkworm larvae through dual promoters of Pph and Pp10. Miao, Yun-Gen,Yue, Wan-Fu,Li, Xing-Hua,Roy, Bhaskar,Li, Guang-Li,Liu, Jian-Mei,Wu, Xiao-Feng,Miao, Yun-Gen,Wu, Wei-Cheng,Zhou, Ji-Yong,Zhang, Chuan-Xi,David, Wan Chi Cheong.

作者其他论文更多>>

Lactic Acid Bacteria Exopolysaccharides Unveiling Multifaceted Insights from Structure to Application in Foods and Health Promotion

作者：Liu, Wei;Wei, Yajun;Yang, Xi;Xiang, Rong;Dong, Bo

关键词：lactic acid bacteria; exopolysaccharides; biological functions; food applications; health benefits; biosynthesis
A Viral RNA Silencing Suppressor Modulates Reactive Oxygen Species Levels to Induce the Autophagic Degradation of Dicer-Like and Argonaute-Like Proteins

作者：Zhai, Shiyu;Pang, Tianxing;Peng, Shiyu;Kang, Zhensheng;Andika, Ida Bagus;Sun, Liying;Zhai, Shiyu;Pang, Tianxing;Peng, Shiyu;Kang, Zhensheng;Andika, Ida Bagus;Sun, Liying;Zou, Shenshen;Deng, Zhiping;Suzuki, Nobuhiro;Sun, Liying

关键词：argonaute; autophagic degradation; cryphonectria hypovirus 1; dicer; reactive oxygen species; RNA silencing suppressor
Whole-transcriptome sequencing reveals the global molecular responses and ceRNA regulatory network involved in programmed cell death of rice cultivars zyk639 and zyk-lm

作者：Zhang, Haipeng;Zheng, Han;Zhao, Hongyu;Wang, Chengyu;Zhang, Haipeng;Zheng, Han;Liang, Weifang;Zhao, Hongyu;Chen, Jianping;Yang, Yong;Zhang, Junjie;Li, Jianzhong;Zhou, Yujie;Xie, Liujie;Yu, Chulang;Chen, Jianping;Dai, Binfeng;Zhong, Liequan;Hou, Fan

关键词：PCD; Whole-transcriptome; LncRNA; WGCNA; ce-RNA
Microbial carbon and phosphorus metabolism regulated by C:N:P stoichiometry stimulates organic carbon accumulation in agricultural soils

作者：Wang, Xiangxiang;Zhang, Hongrui;Cao, Dan;Wei, Liang;Wang, Shuang;Ding, Jina;Chen, Jianping;Ge, Tida;Zhu, Zhenke;Wang, Xiangxiang;Zhang, Hongrui;Cao, Dan;Wu, Cuiyan;Wang, Xianting;Guo, Bin;Chen, Hao

关键词：Soil nutrients; Microbial metabolism; Ecoenzymatic stoichiometry; Microbial carbon use efficiency
Low-dose of oligosaccharins boosts antiviral immunity through induction of multiple defense pathways in rice

作者：Wang, Guoda;Lu, Mingmin;He, Qianqian;Du, Juan;Li, Lulu;Zhang, Hehong;Wei, Zhongyan;Lu, Yuwen;Chen, Jianping;Sun, Zongtao;Li, Yanjun;Song, Wen

关键词：Oligosaccharins; Plant immunity; Jasmonic acid (JA); Reactive oxygen species (ROS); Antiviral defense; Southern rice black-streaked dwarf virus (SRBSDV); Low-dose
Glycerol-derived reuterin regulates human intestinal microbiota and metabolites

作者：Yang, Xi;Yi, Hongbo;Mori, Hirotada;Liu, Wei;Zhang, Xiaoling;Yang, Qiao;Sun, Minhua;Liao, Shenquan;Xiang, Rong;Zhang, Hao

关键词：reuterin; antimicrobe; gut microbiota; intestinal gas production; SCFA
Identification and Genome Sequencing of Novel Virulent Strains of Xanthomonas oryzae pv. oryzae Causing Rice Bacterial Blight in Zhejiang, China

作者：Liang, Weifang;Chen, Jianping;Liang, Weifang;Zhou, Yuhang;Chen, Xinyu;Chen, Jianping;Yang, Yong;Zhou, Yuhang;Xu, Zhongtian;Li, Yiyuan;Yu, Chulang;Chen, Jianping;Chen, Xinyu;Hou, Fan;Dai, Binfeng;Zhong, Liequan;Bi, Ji-An;Yan, Chengqi;Xie, Liujie

关键词：avirulence gene; genome sequencing; TAL effector; Xanthomonas oryzae pv. oryzae

Nucleo-cytoplasmic shuttling of VPg encoded by Wheat yellow mosaic virus requires association with the coat protein

作者其他论文 更多>>

Lactic Acid Bacteria Exopolysaccharides Unveiling Multifaceted Insights from Structure to Application in Foods and Health Promotion

A Viral RNA Silencing Suppressor Modulates Reactive Oxygen Species Levels to Induce the Autophagic Degradation of Dicer-Like and Argonaute-Like Proteins

Whole-transcriptome sequencing reveals the global molecular responses and ceRNA regulatory network involved in programmed cell death of rice cultivars zyk639 and zyk-lm

Microbial carbon and phosphorus metabolism regulated by C:N:P stoichiometry stimulates organic carbon accumulation in agricultural soils

Low-dose of oligosaccharins boosts antiviral immunity through induction of multiple defense pathways in rice

Glycerol-derived reuterin regulates human intestinal microbiota and metabolites

Identification and Genome Sequencing of Novel Virulent Strains of Xanthomonas oryzae pv. oryzae Causing Rice Bacterial Blight in Zhejiang, China

意 见 箱

作者其他论文更多>>

意见箱