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Changes in protein composition of epidermal mucus in turbot Scophthalmus maximus (L.) under high water temperature

文献类型： 外文期刊

作者： Ma Ai-Jun 1 ; Huang Zhi-hui 1 ; Wang Xin-An 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China

关键词： Turbot (Scophthalmus maximus);Skin mucous protein;Two-dimensional gel electrophoresis (2-DE) MALDI-TOF-TOF;Protein marker

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To explore the skin mucous of juvenile turbot (Scophthalmus maximus) protein under thermo-treatment in different temperatures (20, 23, 25, and 27 A degrees C), the corresponding proteome maps were constructed by two-dimensional gel electrophoresis (2-DE), from which the peptide mass map with MALDI-TOF-TOF was obtained, and a novel protein of polypeptide was identified by database retrieval. Results show that the proteome maps varied remarkably with temperature, indicating the increase or decrease in protein spot. Totally, 209 protein spots were matched in five maps in temperature using ImageMaster 2D Platinum 6.0. In addition, six unique protein spots were selected and identified with MALDI-TOF-TOF. By searching database for protein identification and function prediction, five proteins were confirmed, of which lectin and cytokeratin are highly potential as protein marker for further research. The information should be useful for better understanding the role of mucus as a component of innate immune system and for identifying genotypes that suit best to the aquiculture environment. These proteins could be used as potential biomarkers to environmental stressors in mucus for providing early warning when fish suffers in a dangerous situation.

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