MiR-125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK-independent pathways
文献类型： 外文期刊
作者： Wu, N. 1 ; Lin, X. 2 ; Zhao, X. 3 ; Zheng, L. 4 ; Xiao, L. 1 ; Liu, J. 1 ; Ge, L. 1 ; Cao, S. 6 ;
作者机构： 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China
3.Qingdao Univ, Cent Lab, Affiliated Hosp, Coll Med, Qingdao 266003, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
5.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China
6.Roswell Pk Canc Inst, Dept Med, Buffalo, NY 14263 USA
期刊名称：BRITISH JOURNAL OF CANCER （ 影响因子：7.64； 五年影响因子：7.57 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Background: We have recently identified miR-125b upregulation in glioblastoma (GMB). The aim of this study is to determine the correlation between miR-125b expression and malignant grades of glioma and the genes targeted by miR-125b. Methods: Real-time PCR was employed to measure the expression level of miR-125b. Cell viability was evaluated by cell growth and colony formation in soft-agar assays. Cell apoptosis was determined by Hoechst 33342 staining and AnnexinV-FITC assay. The Luciferase assay was used to confirm the actual binding sites of p38MAPK mRNA. Western blot was used to detect the gene expression level. Results: The expression level of miR-125b is positively correlated with the malignant grade of glioma. Ectopic expression of miR-125b promotes the proliferation of GMB cells. Knockdown of endogenous miR-125b inhibits cell proliferation and promotes cell apoptosis. Further studies reveal that p53 is regulated by miR-125b. However, downregulation of the endogenous miR-125b also results in p53-independent apoptotic pathway leading to apoptosis in p53 mutated U251 cells and p53 knockdown U87 cells. Moreover, p38MAPK is also regulated by miR-125b and downregulation of miR-125b activates the p38MAPK-induced mitochondria apoptotic pathway. Conclusion: High-level expression of miR-125b is associated with poor outcomes of GMB. MiR-125b may have an oncogenic role in GMB cells by promoting cell proliferation and inhibiting apoptosis.
- 相关文献
作者其他论文 更多>>
-
BIOMASS AND SPATIAL DISTRIBUTION ESTIMATES OF HAIRTAIL (TRICHIURUS JAPONICUS) IN THE NORTHERN WATERS OF TAIWAN
作者：Kang, M.;Fajaryanti, R.;Huang, H.;Ye, H.;Zhang, H.;Liu, J.
关键词：hairtail; biomass; fish school property; water temperature; fishing gear
-
A post-processing method to remove interference noise from acoustic data collected from Antarctic krill fishing vessels
作者：Wang, X.;Wang, X.;Zhang, J.;Zhao, X.;Zhao, X.
关键词：
-
The ontogeny of the retina of Chinese sturgeon (Acipenser sinensis)
作者：Chai, Y.;Xie, C.;Wei, Q.;Chen, X.;Liu, J.
关键词：
-
Large-scale cultivation of fingerlings of the Chinese Sturgeon Acipenser sinensis for re-stocking: a description of current technology
作者：Zhu, Y.;Wei Q.;Yang, D.;Wang, K.;Chen, X.;Liu, J.;Li, L.
关键词：
-
On-site determination of Pb2+ and Cd2+ in seawater by double stripping voltammetry with bismuth-modified working electrodes
作者：Zhang, X.;Zhang, Y.;Ding, D.;Zhao, J.;Qu, K.;Liu, J.;Yang, W.
关键词：Heavy metals;On-site determination;Double stripping voltammetry;Bi film;Seawater;Environmental monitoring
-
Non-lethal gill biopsy of juvenile hybrid sturgeon (Acipenser ruthenus female x A. schrenckii male): validity and impact on growth and osmoregulation
作者：Zhao, F.;Zhuang, P.;Zhang, T.;Zhang, L.;Liu, J.;Hou, J.
关键词：
-
Effects of lateral morphology on swimming performance in two sturgeon species
作者：Qu, Y.;Duan, M.;Yan, J.;Feng, G.;Liu, J.;Zhang, L.;Zhuang, P.;Duan, M.;Qu, Y.;Yan, J.;Zhuang, P.
关键词：
