您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Activation of P27kip1-cyclin D1/E-CDK2 pathway by polysaccharide from Phellinus linteus leads to S-phase arrest in HT-29 cells

文献类型： 外文期刊

作者： Zhong, Shi 1 ; Ji, Dong-Feng 1 ; Li, You-Gui 1 ; Lin, Tian-Bao 1 ; Lv, Zhi-Qiang 1 ; Chen, Hua-Ping 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

2.Hangzhou Qing Zheng Biol Sci Technol Co, Hangzhou 311700, Zhejiang, Peoples R China

关键词： Cyclins/CDK complexes;P27kip1;Polysaccharide;S-phase arrest

期刊名称：CHEMICO-BIOLOGICAL INTERACTIONS （ 影响因子：5.192； 五年影响因子：4.61 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Our previous study showed that polysaccharide (P1) from Phellinus linteus exhibits a significant inhibitive activity on human colorectal carcinoma cells (HT-29). However its novel molecular mechanism remains unknown. To obtain insights into P1's mechanism of action, we examined its effects on cell proliferation in vitro and in vivo, cell cycle distribution, apoptosis, autophagy, and expression of several cell cycle interrelated proteins in HT-29 cells. Interestingly, we found that volume and weight of the solid tumor significantly decreased in P1 (200 mg/kg)-treated mice compared with the control. However, slightly increased the body weight of the P1 treated tumor-bearing mice, with no significant increased ALT, AST levels in serum and LPO concentration in liver and kidney indicated that P1 has no toxicity to mammals at a dose of 200 mg/kg. Furthermore, P1 caused a significantly dose-dependent increase in the S-phase cell cycle, but no apoptosis and autophagy in HT-29 cells. RT-PCR and Western blot results showed significantly down-regulated expressions of cyclin D1, cyclin E, and CDK2, as well as increased expressions of P27kip1 in P1 (100 μg/mL)-treated HT-29 cells. These results suggested that the activation of P27kip1-cyclin D1/E-CDK2 pathway is involved in P1-induced S-phase cell cycle arrest in HT-29 cells.

  • 相关文献

[1]Polysaccharide from Phellinus linteus induces S-phase arrest in HepG2 cells by decreasing calreticulin expression and activating the P27kip1-cyclin A/D1/E-CDK2 pathway. Li, You-Gui,Ji, Dong-Feng,Zhong, Shi,Liu, Pei-Gang,Lv, Zhi-Qiang,Zhu, Jian-Xun,Chen, Jin-E,Chen, Hua-Ping. 2013

[2]Protocatechualdehyde Induces S-Phase Arrest and Apoptosis by Stimulating the p27(KIP1)-Cyclin A/D1-CDK2 and Mitochondrial Apoptotic Pathways in HT-29 Cells. Zhong, Shi,Li, You-Gui,Ji, Dong-Feng,Lin, Tian-Bao,Lv, Zhi-Qiang.

[3]Hybrid of 1-deoxynojirimycin and polysaccharide from mulberry leaves treat diabetes mellitus by activating PDX-1/insulin-1 signaling pathway and regulating the expression of glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase in alloxan-induced diabetic mice. Li, You-Gui,Ji, Dong-Feng,Zhong, Shi,Lv, Zhi-Qiang,Lin, Tian-Bao,Chen, Shi,Hu, Gui-Yan. 2011

[4]Hypoglycemic effect of deoxynojirimycin-polysaccharide on high fat diet and streptozotocin-induced diabetic mice via regulation of hepatic glucose metabolism. Li, You-gui,Ji, Dong-feng,Zhong, Shi,Lin, Tian-bao,Lv, Zhi-qiang.

[5]Comparison of the preliminary characterizations and antioxidant properties of polysaccharides obtained from Phellinus baumii growth on different culture substrates. Zhang Zuo-fa,Lv Guo-ying,Song Ting-ting,Jin Qun-li,Fan Lei-fa,Cai Wei-ming,Huang Jian-bo.

[6]Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes. Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Fan, Leifa,He, Weiqiang,Shi, Liangen.

[7]Extraction optimization and biological properties of a polysaccharide isolated from Gleoestereum incarnatum. Zhang, Zuo-fa,Lv, Guo-ying,Cheng, Jian-hui,Fan, Lei-fa,Jiang, Xue.

[8]Antioxidant and DNA damage protecting potentials of polysaccharide extracted from Phellinus baumii using a delignification method. Jin, Qun-li,Zhang, Zuo-fa,Lv, Guo-ying,Cai, Wei-ming,Fan, Lei-fa,Cheng, Jun-wen,Wang, Jian-gong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台