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The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor

文献类型： 外文期刊

作者： Sun, Liying 1 ; Andika, Ida Sagus 1 ; Shen, Jiangfeng 2 ; Yang, Di 3 ; Ratti, Claudio 4 ; Chen, Jianping 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, MoA Key Lab Plant Protect & Biotechnol, Zhejiang Prov Key Lab Plant Virol,Inst Virol & Bi, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China

3.Anhui Agr Univ, Coll Life Sci, Hefei 230036, Peoples R China

4.Univ Bologna, DipSA Plant Pathol, I-40127 Bologna, Italy

关键词： Alternative translation initiation;Chinese wheat mosaic virus;CUG codon;Cysteine-rich suppressor;Furovirus;Larger form coat protein

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： Some viruses use alternative translation initiation at non-AUG codons as a strategy to produce multiple proteins during gene expression. Here we show that, using this strategy, Chinese wheat mosaic virus (CWMV; Furovirus) expresses a larger form of coat protein (N-ext/CP) in infected plants. Site-directed mutagenesis and transient expression analysis confirmed that CWMV N-ext/CP is initiated at an upstream in-frame CUG codon at nucleotide position 207-209 of RNA 2, which adds a 39 amino acid (aa) N-terminal extension to the major CP. Interestingly, in planta and in vitro analyses indicated that CWMV N-ext/CP but not CP interacts with the CWMV cysteine-rich protein (CRP), an RNA silencing suppressor. We further determined that the N-terminal 39 aa extension, particularly the 10 aa region immediately upstream of the major CP coding region is responsible for the interaction of N-ext/CP with CRP. In an Agrobacterium co-infiltration assay, co-expression with N-ext/CP did not affect CRP silencing suppression activity. Thus the alternative translation initiation at a CUG codon provides the CWMV N-ext/CP with the ability to bind to the viral silencing suppressor.

  • 相关文献

[1]Sequence of a second isolate of Chinese wheat mosaic furovirus. Yang, J,Chen, J,Chen, J,Jiang, H,Zhao, Q,Adams, MJ. 2001

[2]Endoplasmic reticulum export and vesicle formation of the movement protein of Chinese wheat mosaic virus are regulated by two transmembrane domains and depend on the secretory pathway. Zheng, Shiling,Zhou, Xueping,Andika, Ida Bagus,Sun, Liying,Chen, Jianping,Tan, Zilong,Kondo, Hideki.

[3]Sequence analysis of a soil-borne wheat mosaic virus isolate from Italy shows that it is the same virus as European wheat mosaic virus and Soil-borne rye mosaic virus. Yang, JP,Chen, JP,Chen, JO,Cheng, Y,Adams, MJ. 2001

[4]Use of monoclonal antibodies for the serological differentiation of wheat and oat furoviruses. Ye, R,Lu, X,Gao, ZZ,Yang, JP,Chen, J,Chen, JP,Adams, MJ,Yu, SQ. 2000

[5]Cloning and expression of the Chinese wheat mosaic virus RNA2 coat protein readthrough and 19 ku cysteine-rich domains and localization of these proteins. Xu, L,Chen, JP,Ye, R,Zhao, XY,Yu, SQ. 2002

[6]Characterization and partial sequence of a new furovirus of wheat in China. Ye, R,Zheng, T,Chen, J,Diao, A,Adams, MJ,Yu, S,Antoniw, JF.

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