Preparation of a Novel Amino-Phosphate Zwitterionic Stationary Phase for Hydrophilic Interaction Chromatography
文献类型： 外文期刊
作者： Cheng, Xiao-Dong 1 ; Peng, Xi-Tian 1 ; Yu, Qiong-Wei 1 ; Yuan, Bi-Feng 1 ; Feng, Yu-Qi 1 ;
作者机构： 1.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ, Dept Chem, Wuhan 430072, Peoples R China
2.Hubei Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Wuhan 430064, Peoples R China
关键词： Hydrophilic interaction chromatography;Zwitterionic stationary phase;Retention mechanism;Polar compounds
期刊名称：CHROMATOGRAPHIA （ 影响因子：2.044； 五年影响因子：1.725 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： A new stationary phase which contains both negatively charged phosphate groups and positively charged amino groups was successfully synthesized by modification of amino-functionalized silica particles with trichlorophosphine oxide (POCl_3) for hydrophilic interaction chromatography (HILIC). The composition of the surface grafts was determined by Fourier transform infrared spectroscopy and elemental analysis. Various parameters, such as column temperature, water content, pH values and ionic strength of the mobile phase were investigated to study the retention mechanism. The results demonstrated that the stationary phase involved a complex retention process including surface adsorption, partitioning and electrostatic interactions. Under optimized conditions, the separation of nucleobases and nucleosides, water-soluble vitamins, organic acids on the novel stationary phase could be achieved in the HILIC mode.
- 相关文献
作者其他论文 更多>>
-
Preparation, Characterization, and Chromatographic Evaluation of Nickel Oxide Deposited Silica Stationary Phase in Hydrophilic Interaction Liquid Chromatography
作者：Guo, Ying-Jie;Yu, Qiong-Wei;Feng, Yu-Qi;Peng, Xi-Tian
关键词：Coordination; Hydrophilic interaction liquid chromatography; Nickel oxide deposited silica; Stationary phase
-
Application of a novel nylon needle filter-based solid-phase extraction device to determination of 1-hydroxypyrene in urine
作者：Yue, Shi-Wen;Zhou, Yi-Lian;Zhao, Qin;Peng, Xi-Tian
关键词：1-hydroxypyrene; nylon needle filter; solid-phase extraction; urine
-
GC-MS-based untargeted metabolomics reveals the key volatile organic compounds for discriminating grades of Yichang big-leaf green tea
作者：Yin, Xiao-Li;Fu, Wen-Jing;Zhou, Ran-Feng;Sun, Weiqing;Ding, Baomiao;Chen, Ying;Gu, Hui-Wen;Zhou, Ran-Feng;Peng, Xi-Tian
关键词：Headspace gas chromatography coupled to; mass spectrometry; Volatile organic compounds; Multivariate statistical analysis; Chinese green tea; Untargeted metabolomics
-
Rapid and Sensitive Detection of Aflatoxin B1, B2, G1 and G2 in Vegetable Oils Using Bare Fe3O4 as Magnetic Sorbents Coupled with High-Performance Liquid Chromatography with Fluorescence Detection
作者：Zhang, Hui-Xian;Zhou, You-Xiang;Peng, Xi-Tian;Zhang, Ping;Fu, Xiao-Fang
关键词：
-
Rapid determination of organophosphorus pesticides in edible vegetable oils by direct microextraction using magnetic mesoporous silica microspheres
作者：Wu, Jian-Yuan;Zhang, Hui-Xian;Peng, Xi-Tian
关键词：magnetic mesoporous silica; magnetic solid phase extraction; matrix effect; organophosphorus pesticides
-
Rapid and sensitive detection of the phenoxy acid herbicides in environmental water samples by magnetic solid-phase extraction combined with liquid chromatography-tandem mass spectrometry
作者：Peng, Mao-Min;Xia, Hong;Hu, Xi-Zhou;Zhou, You-Xiang;Peng, Li-Jun;Peng, Xi-Tian;Han, Ya-Quan;Peng, Xi-Tian
关键词：liquid chromatography; magnetic solid-phase extraction; multiwalled carbon nanotubes; phenoxy acid herbicides; tandem mass spectrometry Institute
-
Rapid and sensitive detection of abamectin in edible oils by solid phase extraction combined with ultra-high-pressure liquid chromatography-tandem mass spectrometry
作者：Wu, Jian-Yuan;Wu, Jian-Yuan;Zhang, Yu;Peng, Xi-Tian;Yu, Qiong-Wei;Feng, Yu-Qi;Peng, Xi-Tian
关键词：
