您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

文献类型： 外文期刊

作者： Li, Wei 1 ; Sun, Wen-xiu 1 ; Meng, Liang 2 ; Hong, Deng-wei 1 ;

作者机构： 1.Yangtze Univ, Dept Biotechnol, Coll Life Sci, Jinzhou 434025, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China

关键词： swamp eel;Monopterus albus;MHC IIA;gene cloning and expression

期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.139 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) class II molecules play an important role in the immune response of vertebrates. In this paper, full-length MHC IIA cDNA was isolated from swamp eel (Monopterus albus) by rapid amplification of cDNA ends PCR. The genomic structure, molecular polymorphism, tissue distribution, and immune response of the MHC IIA gene to bacterial challenge were investigated. The full-length cDNA (GenBank accession No.: KC616308) is 1,509 bp in length including an 83 bp-long 5' untranslated region (UTR) and a 709 bp-long 3' UTR, which encoded a 238 amino acids protein. In the 2,339 bp-long MHC IIA genomic DNA, four exons and three introns were identified. Sequence comparison exhibited that the deduced amino acid sequence shared 27.1-66.3% identity with those of other species. Seven alleles were identified from five healthy individuals. Number of alleles per individual diversified from two to five. Five different 5' UTR sequences and two different 3' UTR sequences from one individual may infer the existence of five loci at least. Real-time quantitative PCR demonstrated that swamp eel MHC IIA transcripts were ubiquitously expressed in ten tissues, but the expression level was distinctly different. Significant changes were observed in liver, spleen, kidney and intestine after challenged with pathogenic bacteria Aeromonas hydrophilia.

  • 相关文献

[1]Androgen rather than estrogen up-regulates brain-type cytochrome P450 aromatase (cyp19a1b) gene via tissue-specific promoters in the hermaphrodite teleost ricefield eel Monopterus albus. Zhang, Yang,Zhang, Shen,Zhou, Wenliang,Lin, Haoran,Zhang, Weimin,Zhang, Lihong,Lin, Haoran,Zhang, Weimin,Ye, Xing,Ge, Wei,Cheng, Christopher H. K.. 2012

[2]Cloning, structure, and expression pattern of the P-450 aromatase gene in rice field eel (Monopterus albus). Yu, Ju-Hua,Tang, Yong-Kai,Li, Jian-Lin. 2008

[3]Pharmacokinetics and residue depletion of praziquantel in rice field eels Monopterus albus. Xu, Ning,Dong, Jing,Yang, Yibin,Ai, Xiaohui,Xu, Ning,Dong, Jing,Xu, Ning,Dong, Jing,Ai, Xiaohui.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台