您好,欢迎访问江苏省农业科学院 机构知识库!

Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library

文献类型: 外文期刊

作者: Xia, Zhengjun 1 ; Wu, Hongyan 1 ; Watanabe, Satoshi 3 ; Harada, Kyuya 3 ;

作者机构: 1.Chinese Acad Sci, Key Lab Soybean Mol Design Breeding, Northeast Inst Geog & Agroecol, Harbin 150081, Peoples R China

2.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

3.Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan

关键词: BAC;Pooling;Screening;Non-gridded library;Soybean

期刊名称:ANALYTICAL BIOCHEMISTRY ( 影响因子:3.365; 五年影响因子:3.049 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.

  • 相关文献

[1]Construction of scFv phage display library with hapten-specific repertories and characterization of anti-ivermectin fragment isolated from the library. Zhang, Xiao,Zhang, Cunzheng,Liu, Yuan,Yu, Xiangyang,Liu, Xianjin. 2010

[2]Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation. Li, Dequan,Nie, Fengya,Wei, Lihui,Wei, Benqiang,Chen, Zhiyi.

[3]Purification and characterization of beta-glucosidase from newly isolated Aspergillus sp MT-0204. Qi, Bin,Liu, Xianjin,Qi, Bin,Wang, Limei. 2009

[4]The Soybean Basic Helix-Loop-Helix Transcription Factor ORG3-Like Enhances Cadmium Tolerance via Increased Iron and Reduced Cadmium Uptake and Transport from Roots to Shoots. Xu, Zhaolong,Liu, Xiaoqing,He, Xiaolan,Xu, Ling,Huang, Yihong,Shao, Hongbo,Zhang, Dayong,Shao, Hongbo,Tang, Boping,Ma, Hongxiang. 2017

[5]A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress. Zhang, Dayong,He, Xiaolan,Xu, Zhaolong,Xu, Ling,Wei, Peipei,Huang, Yihong,Brestic, Marian,Ma, Hongxiang,Shao, Hongbo,Tong, Jinfeng,Brestic, Marian,Shao, Hongbo. 2016

[6]GmHKT1;4, a novel soybean gene regulating Na+/K+ ratio in roots enhances salt tolerance in transgenic plants. Chen, Huatao,Chen, Xin,Gu, Heping,Wu, Bingyue,Zhang, Hongmei,Yuan, Xingxing,Cui, Xiaoyan. 2014

[7]Whole-genome identification and expression analysis of K+ efflux antiporter (KEA) and Na+/H+ antiporter (NHX) families under abiotic stress in soybean. Chen Hua-tao,Chen Xin,Wu Bing-yue,Yuan Xing-xing,Zhang Hong-mei,Cui Xiao-yan,Liu Xiao-qing. 2015

[8]Over-expression of GmMYB39 leads to an inhibition of the isoflavonoid biosynthesis in soybean (Glycine max. L). Liu, Xiaoqing,Xu, Ling,Xu, Zhaolong,Huang, Yihong,He, Xiaolan,Ma, Hongxiang,Yi, Jinxin,Zhang, Dayong,Yuan, Lingling.

[9]Genome-wide analysis of terpene synthases in soybean: Functional characterization of GmTPS3. Huang, Fang,Wang, Xia,Wang, Jiao,Yu, Deyue,Liu, Jianyu,Zhang, Man,Zheng, Rui.

[10]Soybean C2H2-Type Zinc Finger Protein GmZFP3 with Conserved QALGGH Motif Negatively Regulates Drought Responses in Transgenic Arabidopsis. Zhang, Dayong,Xu, Zhaolong,Wei, Peipei,Xu, Ling,Wan, Qun,Huang, Yihong,He, Xiaolan,Shao, Hongbo,Ma, Hongxiang,Tong, Jinfeng,Yang, Jiayin,Shao, Hongbo. 2016

[11]Molecular identification of genes controlling flowering time, maturity, and photoperiod response in soybean. Xia, Zhengjun,Zhai, Hong,Liu, Baohui,Kong, Fanjiang,Yuan, Xiaohui,Wu, Hongyan,Xia, Zhengjun,Cober, Elroy R.,Harada, Kyuya. 2012

[12]Over-expression of GmWRKY111 Enhances NaCl Tolerance of Salt-Sensitive Genotype of Glycine max. Xu, Zhao Long,Ma, Hong Xiang,Xu, Zhao Long,Yi, JinXin,Xu, Ling,Liu, Xiao Qing,He, XiaoLan,Huang, Yi Hong,Ma, Hong Xiang,Zhang, Da Yong,Ali, Zulfiqar,Karim, Ihsan,Rasul, Maria,Ali, Zulfiqar,Karim, Ihsan,Rasul, Maria. 2014

[13]Validation and high-resolution mapping of a major quantitative trait locus for alkaline salt tolerance in soybean using residual heterozygous line. Tuyen, D. D.,Zhang, H. M.,Xu, D. H.,Tuyen, D. D.,Zhang, H. M..

[14]Identification of novel loci for salt stress at the seed germination stage in soybean. Kan, Guizhen,Li, Yakai,Hu, Zhenbin,Zhang, Wei,He, Xiaohong,Yu, Deyue,Ning, Lihua.

[15]Heterologous expression of GmSIP1;3 from soybean in tobacco showed and growth retardation and tolerance to hydrogen peroxide. Zhang, Dayong,Huang, Yihong,Wan, Qun,Xu, Zhaolong,Shao, Hongbo,Shao, Hongbo,Kumar, Manoj,Pandey, Girdhar K..

作者其他论文 更多>>