Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library
文献类型: 外文期刊
作者: Xia, Zhengjun 1 ; Wu, Hongyan 1 ; Watanabe, Satoshi 3 ; Harada, Kyuya 3 ;
作者机构: 1.Chinese Acad Sci, Key Lab Soybean Mol Design Breeding, Northeast Inst Geog & Agroecol, Harbin 150081, Peoples R China
2.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China
3.Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan
关键词: BAC;Pooling;Screening;Non-gridded library;Soybean
期刊名称:ANALYTICAL BIOCHEMISTRY ( 影响因子:3.365; 五年影响因子:3.049 )
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收录情况: SCI
摘要: Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.
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