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Structural elucidation and in vitro antitumor activity of a novel oligosaccharide from Bombyx batryticatus

文献类型： 外文期刊

作者： Jiang, Xue 1 ; Zhang, Zuofa 2 ; Chen, Yajie 1 ; Cui, Zhenteng 1 ; Shi, Liangen 1 ;

作者机构： 1.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Bombyx batryticatus;Oligosaccharide;Structural elucidation;Antitumor activity

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

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收录情况： SCI

摘要： An oligosaccharide BBPW-2 was isolated and purified from Bombyx batryticatus, its molecular weight was 2.0 x 10~3 Da, and its structure was elucidated by compositional, methylation and NMR analysis. Our results showed that BBPW-2 consisted of β-D-( 1→ 2,6)-glucopyranose and β-d-( 1→2,6)-mannosyl units serving as the backbone, α-D-(l → 2)-galactopyranose and α-D-( 1 → 3)-mannosyl units as branches, and α-D-Manp and β-D-Glcp as terminals. The in vitro inhibitory activity of BBPW-2 was measured using MTT and and crystal violet assays, which suggested that BBPW-2 had direct cytotoxic effects on the cancer cell lines HeLa and HepG2 (particularly HeLa cells), and had a long-term antiproliferative effect on MCF-7 cells, respectively. Apoptosis and cellcycle analysis of HeLa cells showed that BBPW-2 induced cellcycle disruption in the G0/G1 and G2/M phases accompanied by an impressive increment of early apoptotic cells and late apoptotic and necrotic cells. These results suggest that BBPW-2 could be a potential chemotherapeutic drug and its antitumor effects deserve further study.

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