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Identification of differentially expressed genes in hepatopancreas of oriental river prawn, Macrobrachium nipponense exposed to environmental hypoxia

文献类型: 外文期刊

作者: Sun, Shengming 1 ; Xuan, Fujun 2 ; Ge, Xianping 1 ; Fu, Hongtuo 1 ; Zhu, Jian 1 ; Zhang, Shiyong 3 ;

作者机构: 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Wuxi 214081, Peoples R China

2.Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Peoples R China

3.Nanjing Agr Univ, Wuxi Fishery Coll, Wuxi 214081, Peoples R China

关键词: Hemocyanin;Hepatopancreas;Hypoxia;Macrobrachium nipponense;Transcriptome

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Hypoxia represents a major physiological challenge for prawn culture, and the hepatopancreas plays an important role in these processes. Here, we applied high-throughput sequencing technology to detect the gene expression profile of the hepatopancreas in Macrobrachium nipponense in response to hypoxia for 3. h and hypoxia for 24. h. Gene expression profiling identified 1925 genes that were significantly up- or down-regulated by dissolved oxygen availability. Functional categorization of the differentially expressed genes revealed that oxygen transport, electron transport chain, reactive oxygen species generation/scavenging, and immune response were the differentially regulated processes occurring during environmental hypoxia. Finally, quantitative real-time polymerase chain reaction using six genes independently verified the tag-mapped results. Immunohistochemistry analysis revealed, for the first time, hemocyanin protein expression as significant hypoxia-specific signature in prawns, which opens the way for in depth molecular studies of hypoxia exposure. The analysis of changes in hepatic gene expression in oriental river prawn provides a preliminary basis for a better understanding of the molecular response to hypoxia exposures.

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