A novel rhizobacterium Bk7 for biological control of brown sheath rot of rice caused by Pseudomonas fuscovaginae and its mode of action
文献类型： 外文期刊
作者： Kakar, Kaleem Ullah 1 ; Duan, Y. -P.; Nawaz, Zarqa; Sun, Guochang; Almoneafy, Abdlwareth A. 1 ; Hassan, M. 1 ;
作者机构： 1.Zhejiang Univ, State Key Lab Rice Biol, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Univ, Key Lab Mol Biol Crop Pathogens & Insects, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China
3.Zhejiang Univ, Key Lab Mol Biol Crop Pathog
关键词： Antimicrobial peptide coding genes;Biofilm formation;Brown sheath rot of rice;Multiplex PCR;RT-qPCR
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）
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收录情况： SCI
摘要： The study presents the first report on biocontrol of brown sheath rot disease of rice caused by Pseudomonas fuscovaginae using rhizo-bacterial isolate Bacillus amyloliquefaciens Bk7. Four potential bioactive antagonists were selected from 120 Bacillus isolates. Results obtained from in vitro laboratory assay showed that rhizosphere bacterial strain Bk7 and its metabolites significantly suppressed the growth of Pseudomonas fuscovaginae with 93 % efficacy. In glasshouse experiments, strain Bk7 exhibited biocontrol efficacy of 76.6 % by reducing the disease incidence to 16.9 %, compared to 72.8 % observed in control treatment. In addition, the isolate Bk7 showed the growth promotion efficacy of plant height (GPE, 46.4 %) and fresh weight (GPE, 84.3 %). Characterization of isolate Bk7 revealed its strong capability for biofilm formation, inorganic phosphate solubilization and production of high amounts of Indole-3 acetic acid, siderophores and ammonia in vitro. Results obtained from multiplex PCR assay confirmed the presence of five lipopeptide biosynthetic gene markers (srfAA, fenD, bmyB, bacA and ituC) in the genome of strain Bk7. Moreover, Real-time qPCR of these genes demonstrated that surfactin, iturin and bacylisin coding genes were highly expressed in response to P. fuscovaginae exposure in vitro. Rhizosphere bacterial strain Bk7 was identified as B. amyloliquefaciens strain Bk7 based on the analysis of 16S rDNA internal transcribed spacer sequences and a fatty acid methyl ester analysis. The results obtained from this study showed the potential usefulness of Bk7 as a biocontrol agent in disease control of rice brown sheath rot.
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