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Hepatic Proteome Sensitivity in Rainbow Trout after Chronically Exposed to a Human Pharmaceutical Verapamil

文献类型： 外文期刊

作者： Li, Zhi-Hua 1 ; Li, Ping 1 ; Sulc, Miroslav 3 ; Hulak, Martin 1 ; Randak, Tomas 1 ;

作者机构： 1.Univ S Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, S Bohemian Res Ctr Aquaculture & Biodivers Hydroc, Vodnany 38925, Czech Republic

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Jinzhou 434000, Peoples R China

3.AS CR, Vvi, Inst Microbiol, Prague 14220 4, Czech Republic

期刊名称：MOLECULAR & CELLULAR PROTEOMICS （ 影响因子：5.911； 五年影响因子：5.998 ）

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收录情况： SCI

摘要： Verapamil (VRP), a cardiovascular pharmaceutical widely distributed and persistent in the aquatic environment, has potential toxicity to fish and other aquatic organisms. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the protein patterns that are differentially expressed in liver of rainbow trout exposed to sublethal concentrations of VRP (0.5, 27.0, and 270 mug/liter) for 42 days. Two-dimensional electrophoresis coupled with MALDI-TOF/TOF mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that the expression of six hepatic acidic proteins were markedly altered in the treatment groups compared with the control group; three proteins especially were significantly down-regulated in fish exposed to VRP at environmental related concentration (0.5 mug/liter). These results suggested that the VRP induce mechanisms against oxidative stress (glucose-regulated protein 78 and 94 and protein disulfide-isomer-ase A3) and adaptive changes in ion transference regulation (calreticulin, hyperosmotic glycine-rich protein). Furthermore, for the first time, protein Canopy-1 was found to be significantly down-regulated in fish by chronic exposure to VRP at environmental related levels. Overall, our work supports that fish hepatic proteomics analysis serves as an in vivo model for monitoring the residual pharmaceuticals in aquatic environment and can provide valuable insight into the molecular events in VRP-induced toxicity in fish and other organisms.

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