Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp
文献类型: 外文期刊
作者: Su, Dongxiao 1 ; Ti, Huihui 1 ; Zhang, Ruifen 1 ; Zhang, Mingwei 1 ; Wei, Zhengchen 1 ; Deng, Yuanyuan 1 ; Guo, Jinxin 1 ;
作者机构: 1.Guangdong Acad Agr Sci, Minist Agr, Key Lab Funct Foods, Sericultural & Agri Food Res Inst, Guangzhou 510610, Guangdong, Peoples R China
2.Huazhong Agr Univ, Dept Food Sci & Technol, Wuhan 430070,
关键词: Lychee;Phenolics;Quercetin 3-0-rutinoside-7-O-α-L-rhamnosidase;Antioxidant activity;Cellular antioxidant activity;Oxygen radical absorbance capacity
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Lychee pulp contains phenolic compounds that are strong antioxidants, but the identities of the major antioxidants present are unknown. In the present study, the major contributors to the antioxidant activity of fresh lychee pulp were identified and their cellular antioxidant activities were investigated. Aqueous acetone extracts of lychee pulp were fractionated on polyamide resin, and those fractions with the largest antioxidant and radical scavenging activities were selected using cellular antioxidant activity and oxygen radical absorbance capacity assays. Three compounds that were major contributors to the antioxidant activity in these fractions were obtained by reverse-phase preparative HPLC and identified as quercetin 3-O-rutinoside-7-O-α-L-rhamnosidase (quercetin 3-rut-7-rha), quercetin 3-0-rutinoside (rutin) and (-)-epicatechin using NMR spectroscopy, HMBC, and ES1-MS spectrometry. The concentration of quercetin 3-rut-7-rha was 17.25 mg per 100 g of lychee pulp fresh weight. This is the first report of the identification and cellular antioxidant activity of quercetin 3-rut-7-rha from lychee pulp.
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