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A novel immunosensor for squamous cell carcinoma antigen determination based on CdTe@Carbon dots nanocomposite electrochemiluminescence resonance energy transfer

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文献类型：外文期刊

作者： Li, Shuhuai ¹ ; Luo, Jinhui ¹ ; Yang, Xinfeng ¹ ; Wan, Yao ¹ ; Liu, Chunhua ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Test Ctr, Haikou 571101, Hainan, Peoples R China

关键词： Magnetic nanoparticles;Electrochemiluminescence;Immunosensor;Squamous cell carcinoma antigen;Resonance energy transfer

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （影响因子：7.46；五年影响因子：6.743 ）

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收录情况： SCI

摘要： A novel immunosensor for the detection of squamous cell carcinoma antigen (SCCA) based on magnetic nanoparticles and electrochemiluminescence resonance energy transfer in CdTe@Carbon dots(CdTe@CDs) is presented. First, Fe_3O_4 magnetic nanoparticles were synthesized as a solid support for the primary antibody. A sandwich-type immunoassay was performed on a magnetic force controlled carbon paste electrode via immunoreaction among the CdTe@CDs-labeled secondary antibody, SCCA, and primary antibody on the surface of the magnetic nanoparticles. A CdTe@CDs on the CdTe@CDs- labeled secondary antibody generated luminescence upon electrochemical oxidation, and great signal amplification was achieved because the CDs transferred energy to CdTe. The system was optimized to realize reliable determination of SCCA concentration within the range 0.02-12 ng/mL with a detection limit of 6.3 fg/mL(S/N = 3).

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