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Diversity of Microbial Community in a Paddy Soil with cry1Ac/cpti Transgenic Rice

文献类型： 外文期刊

作者： Song Ya-Na 1 ; Su Jun 1 ; Chen Rui 1 ; Lin Yan 1 ; Wang Feng 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Biol Technol, Fuzhou 350003, Peoples R China

关键词： bacterial community;denaturing gradient gel electrophoresis;fungal community;genetically modified plants;real-time polymerase chain reaction

期刊名称：PEDOSPHERE （ 影响因子：3.911； 五年影响因子：4.814 ）

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收录情况： SCI

摘要： Two types of cry1Ac/cpti transgenic rice (GM1 and GM2) and their parental non-cry1Ac/cpti rice (CK1 and CK2) were planted in the field at Wufeng, Fujian Province, China for four years to investigate the influence of genetically modified rice on diversity of bacterial and fungal community in the paddy soil. The community composition and abundance of bacteria or fungi in the paddy soil were assessed at different growth stages of rice by denaturing gradient gel electrophoresis and real-time polymerase chain reaction based on 16S rRNA gene or SSU rRNA gene in the 4th year after the experimental establishment. The composition of bacterial or fungal community changed during rice growth, while no significant differences were observed between the fields cultivated with GM1 and CK1, or between the fields cultivated with 0M2 and CK2 in either bacterial or fungal community composition. The copy numbers of bacterial 168 rRNA gene in the soils with CK1, CK2, GM1 and GM2 ranged from 5.64 x 10(11) to 6.89 x 10(11) copies g(-1) dry soil at rice growth stages, and those of fungal SSU rRNA gene from 5.24 x 10(8) to 8.68 x 10(8) copies g(-1) dry soil. There were no marked differences in the copies of bacterial 16S rRNA gene or fungal SSU rRNA gene between CK1 and GM1 or between CK2 and GM2 at any growth stage of rice. Planting cry1Ac/cpti transgenic rice had no significant effect on composition and abundance of bacterial and fungal community in paddy soil during the rice growing season at least in the short term.

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