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MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE-BOUND TREHALASES IN THE COTTON MIRID BUG, Apolygus lucorum

文献类型： 外文期刊

作者： Tan, Yongan 1 ; Xiao, Liubin 1 ; Sun, Yang 1 ; Zhao, Jing 1 ; Bai, Lixin 1 ; Xiao, Yingfang 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

2.Univ Florida, Midflorida Res & Educ Ctr, Gainesville, FL 32611 USA

关键词： Apolygus lucorum;soluble trehalase;membrane-bound trehalase;20-hydroxyecdysone

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

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收录情况： SCI

摘要： Trehalose, a major hemolymph sugar in insects, is hydrolyzed by trehalase. We identified a soluble and a membrane-bound form of trehalase and isolated the corresponding mRNA, ALTre-1, and ALTre-2 in the cotton mirid bug, Apolygus lucorum. The deduced amino acid sequences of ALTre-1 and ALTre-2 revealed mature proteins with 643 and 617 amino acids, respectively. ALTre-1 and ALTre-2 contained trehalase signature motifs, and ALTre-2 contained a putative transmembrane domain near the C-terminus, suggesting that ALTre-1 and ALTre-2 encoded a soluble trehalase and a membrane-bound trehalase, respectively. Comparison of trehalase activity at different developmental stages and in six tissues indicated that soluble trehalase activity accounted for the majority of total trehalase activity in A. lucorum. ALTre-1 and ALTre-2 were expressed in all tissues and stages, with the highest expression of both in the second instar nymphs, ALTre-1 in the ovary and malpighian tubules, ALTre-2 in the flight muscles and fat body. Following the exposure of second instar nymph to 20-E, the soluble trehalase activity increased gradually while the membrane-bound trehalase activity remained at its initial level. Similarly, 20-E upregulated ALTre-1 expression but had no effect on ALTre-2 expression. These results suggest that an increase of this soluble trehalase activity was upregulated by ALTre-1 gene.

  • 相关文献

[1]The nuclear hormone receptor E75A regulates vitellogenin gene (Al-Vg) expression in the mirid bug Apolygus lucorum. Tan, Y. -A.,Zhao, X. -D.,Hao, D. -J.,Sun, Y.,Zhao, J.,Jiang, Y. -P.,Bai, L. -X.,Xiao, L. -B.. 2018

[2]Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses. Zhao, Jing,Sheng, Yang,Bai, Li-Xin,Tan, Yongan,Xiao, Liu-Bin,Xu, Guang-Chun,Sun, Yang,Zhang, Yong-Jun,Xiao, Ying-Fang. 2016

[3]Ecdysone receptor isoform-B mediates soluble trehalase expression to regulate growth and development in the mirid bug, Apolygus lucorum (Meyer-Dur). Tan, Y. -A.,Xiao, L. -B.,Zhao, J.,Sun, Y.,Bai, L. -X.,Xiao, Y. -F..

[4]Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dur). Tan, Yong-An,Xiao, Liu-Bin,Zhao, Jing,Sun, Yang,Bai, Li-Xin.

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