Direct electron transfer of hemoglobin intercalated in exfoliated Ni-Al-CO3 layered double hydroxide and its electrocatalysis towards hydrogen peroxide
文献类型： 外文期刊
作者： Liu, Yu-ge 1 ; Zhang, Xiu-mei 1 ; Tu, Xing-hao 1 ; Wei, Chang-bin 1 ; Lv, Ling-ling 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Key Lab Trop Fruit Biol, Minist Agr, Zhanjiang 524091, Peoples R China
期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）
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收录情况： SCI
摘要： In this study, hemoglobin (Hb) was entrapped into the exfoliated Ni-Al-CO3 layered double hydroxides (LDH). UV-vis spectra analysis displayed that no significant denaturation occurred to the protein. Electrochemical results showed that exfoliation of LDH enhanced the direct electron transfer between protein molecules and electrode, and the entrapped protein showed high bioactivity in a wide range of pH values. A pair of well-defined redox peaks was observed at-0.39 and-0.33 V on the glassy carbon electrode (GCE) modified with the Hb-LDH composite. The electrode reactions showed a surface-controlled process with single electron transfer at the scan rate from 100 to 400 mV s~(-10. The sensor constructed displayed an excellent response to the reduction of hydrogen peroxide (H2O2) with a wide linear range, low detection limit and good stability. The modified electrode can also be used for the reduction of oxygen.
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