您好,欢迎访问中国水产科学研究院 机构知识库!

Molecular cloning, tissue distribution and expression analysis of a manganese superoxide dismutase in blunt snout bream Megalobrama amblycephala

文献类型: 外文期刊

作者: Sun, Shengming 1 ; Zhu, Jian 1 ; Jiang, Xiaojun 2 ; Li, Bing 1 ; Ge, Xianping 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Wuxi Fishery Coll, Wuxi 214081, Peoples R China

关键词: Megalobrama amblycephala;Mitochondrial manganese superoxide dismutase;Nitrite;Transcriptional analysis

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The full-length mitochondrial manganese superoxide dismutase cDNA of blunt snout bream Megalobrama amblycephala (denoted as MamMnSOD) was identified in liver using homology cloning and rapid amplification of cDNA ends. The full-length cDNA of MamMnSOD consisted of 986 bp, with an open reading frame encoding 224 amino acids, a 58-bp 5' untranslated region and a 256-bp 3' untranslated region. The deduced amino acid sequences of MamMnSOD showed high sequence homology to mitochondrial MnSODs from crustaceans. Several motifs, including three mitochondrial MnSOD signatures, amino acid residues responsible for coordinating the manganese, and the putative active center, were almost completely conserved in the deduced amino acid sequences of MamMnSOD. The mRNA expression of MamMnSOD in the tissues of heart, liver, spleen, kidney, muscle, intestine, and gill was examined by quantitative real-time PCR; the highest expression was in the liver. Transcription of MamMnSOD was kinetically modulated in response to nitrite stress in liver and gill tissues. The purified recombinant MamMnSOD showed potent antioxidant activity. Polyclonal antibodies generated from the recombinant product of MamMnSOD were used to specifically identify the native protein in liver of M. amblycephala. Collectively, the findings of this study strongly suggested that MamMnSOD combats oxidative stress and cellular damage induced by nitrite, by detoxifying harmful reactive oxygen species in M. amblycephala.

  • 相关文献

[1]Identification and mRNA expression of antioxidant enzyme genes associated with the oxidative stress response in the Wuchang bream (Megalobrama amblycephala Yih) in response to acute nitrite exposure. Sun, Shengming,Ge, Xianping,Zhu, Jian,Xuan, Fujun,Jiang, Xiaojun. 2014

[2]Nitrite-induced hepatotoxicity in Bluntsnout bream (Megalobrama amblycephala): The mechanistic insight from transcriptome to physiology analysis. Sun, Shengming,Ge, Xianping,Zhu, Jian,Xuan, Fujun,Yu, Na.

[3]The characterization, expression and activity analysis of superoxide dismutases (SODs) from Procambarus clarkii. Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Huang, Yanqing. 2013

[4]The Superoxide Dismutase from Red Claw Crayfish, Cherax quadricarinatus: Molecular Cloning and Characterization Analysis. Gu, Wei,Chen, Jing,Hou, Libo,Meng, Qingguo,Wang, Wen,Gu, Wei,Chen, Jing,Hou, Libo,Meng, Qingguo,Wang, Wen,Huang, Yanqing,Xia, Siyao.

[5]Optimization of nitrite reductase production conditions in Lactobacillus plantarum from salted fish. Wang, Yanan,Wu, Yanyan,Yang, Xianqing,Li, Laihao,Qi, Bo. 2013

[6]Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Jia, Rui,Huo, Huan-Huan,Jia, Rui,Lei, Ji-Lin,Liu, Bao-Liang,Huang, Bin,Yin, Shu-Ting,Han, Cen.

[7]The physiological performance and immune response of juvenile turbot (Scophthalmus maximus) to nitrite exposure. Jia, Rui,Lei, Ji-Lin,Han, Cen.

[8]Effect of High Dietary Carbohydrate on Growth, Serum Physiological Response, and Hepatic Heat Shock Protein 70 Expression of Wuchang Bream (Megalobrama amblycephala) at Two Temperatures. Zhou, Chuanpeng,Liu, Bo,Xie, Jun,Ge, Xianping,Liu, Bo,Xie, Jun,Ge, Xianping. 2013

[9]Effects of Dietary Emodin Supplementation on Growth Performance, Non-Specific Immune Responses, and Disease Resistance to Aeromonas hydrophila in Juvenile Wuchang Bream (Megalobrama amblycephala). Zhang, Yuan-yuan,Liu, Bo,Ge, Xian-ping,Xie, Jun,Cui, Yan-ting,Zhang, Yuan-yuan,Liu, Bo,Ge, Xian-ping,Xie, Jun,Ren, Ming-chun,Zhou, Qun-lan,Sun, Sheng-ming,Cui, Yan-ting,Chen, Ru-li,Pan, Liang-kun,Zhang, Yuan-yuan,Liu, Wen-bin. 2014

[10]Regulation mechanism of oxidative stress induced by high glucose through PI3K/Akt/Nrf2 pathway in juvenile blunt snout bream (Megalobrama amblycephala). Pan, Wenjing,Huang, Xin,Ge, Xianping,Moosa, Silli Laban,Liu, Bo,Liang, Hualiang,Zhang, Wuxiao,Miao, Linghong,Lin, Yan,Ge, Xianping,Liu, Bo,Ren, Mingchun,Zhou, Qunlan,Pan, Liangkun. 2017

[11]Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding. Xue Chunyu,Xi Bingwen,Ren Mingchun,Xie Jun,Xu Pao,Xue Chunyu,Dong Jingjing,Xie Jun,Xi Bingwen. 2015

[12]Effects of Antibacterial Peptide Extracted from Bacillus subtilis fmbJ on the Growth, Physiological Response and Disease Resistance of Megalobrama amblycephala. He, Yijin,Liu, Bo,Xie, Jun,Ge, Xianping,Xu, Pao,Lu, Ying,Lu, Fengxia,Lu, Zhaoxin. 2014

[13]Cloning and expression analysis of a heat shock protein 90 beta isoform gene from the gills of Wuchang bream (Megalobrama amblycephala Yih) subjected to nitrite stress. Sun, S. M.,Zhu, J.,Ge, X. P.,Zhang, C. F.,Miao, L. H.,Jiang, X. J.. 2015

[14]Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala). Miao, Ling-Hong,Pan, Wen-Jing,Huang, Xin,Ge, Xian-Ping,Liu, Bo,Miao, Ling-Hong,Lin, Yan,Ge, Xian-Ping,Ren, Ming-Chun,Zhou, Qun-Lan,Liu, Bo. 2017

[15]Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress. Ming, Jianhua,Xie, Jun,Xu, Pao,Ge, Xianping,Ming, Jianhua,Ye, Jinyun,Ming, Jianhua,Liu, Wenbin. 2012

[16]Effects of Dietary Vitamin C on the Physiological Responses and Disease Resistance to Ph Stress and Aeromonas Hydrophila Infection of Megalobrama Amblycephala. Liu, Bo,Wan, Jinjuan,Ge, Xianping,Xie, Jun,Liu, Bo,Ge, Xianping,Xie, Jun,Zhou, Qunlan,Miao, Linghong,Ren, Mingchun,Pan, Liangkun. 2016

[17]Molecular cloning and expression of two HSP70 genes in the Wuchang bream (Megalobrama amblycephala Yih). Ming, Jianhua,Xie, Jun,Xu, Pao,Ge, Xianping,Liu, Bo,He, Yijin,Cheng, Yanfen,Zhou, Qunlan,Pan, Liangkun,Ming, Jianhua,Liu, Wenbin. 2010

[18]The Effect of Muscle-Regulatory Factor Genes and Satellite Cell Response to Recombinant Hsp70 Protein on Megalobrama amblycephala Skeletal Muscle. Zhu, Kecheng,Zhang, Dianchang,Wang, Huanling,Zhu, Kecheng,Zhang, Dianchang,Wang, Huanling. 2016

[19]Effects of Supplemented Dietary Curcumin on Growth and Non-Specific Immune Responses in Juvenile Wuchang Bream (Megalobrama amblycephala). Xia, Si-lei,Ge, Xian-ping,Liu, Bo,Xie, Jun,Miao, Ling-hong,Zhang, Wu-xiao,Jiang, Xiao-jun,Xia, Si-lei,Ge, Xian-ping,Liu, Bo,Xie, Jun,Miao, Ling-hong,Ren, Ming-chun,Zhou, Qun-lan,Zhang, Wu-xiao,Jiang, Xiao-jun,Chen, Ru-li,Pan, Liang-kun. 2015

[20]Oligochitosan stimulated phagocytic activity of macrophages from blunt snout bream (Megalobrama amblycephala) associated with respiratory burst coupled with nitric oxide production. Liu, Lichun,Zhou, Yang,Zhao, Xiaoheng,Wang, Hong,Wang, Li,Yuan, Gailing,Asim, Muhammad,Liu, Xiaoling,Lin, Li,Wang, Weimin,Lin, Li,Wang, Weimin,Lin, Li,Zeng, Lingbing,Lin, Li.

作者其他论文 更多>>