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Seasonal changes and human chorionic gonadotrophin (hCG) effects on innate immune genes expression in goldfish (Carassius auratus)

文献类型: 外文期刊

作者: Zhong, Huan 1 ; Zhou, Yi 2 ; Yu, Fan 4 ; Xiao, Jun 2 ; Gan, Xi 2 ; Zhang, Ming 1 ;

作者机构: 1.Guangxi Univ, Anim Reprod Inst, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China

2.Guangxi Fisheries Res Inst, Guangxi Key Lab Aquat Genet Breeding & Hlth Aquac, Nanning 530021, Guangxi, Peoples R China

3.Changsha Univ, Dept Biotechnol & Environm Sci, Changsha 410003, Hunan, Peoples R China

4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Genet Breeding Aquat Anim, Wuxi 214081, Jiangsu, Peoples R China

关键词: Human chorionic gonadotrophin;Innate immunity;LH;HPG axis;Goldfish

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: We profiled the expression of a group of proinflammatory immune genes, comprising TNF alpha-1, TNF alpha-2, IFN-gamma, IL1 beta-1, IL1 beta-2, CCL-1, and CXCL-8 in liver, head kidney, gills, and spleen of goldfish, during the reproductive cycle and in response to injection of the hormone human chorionic gonadotrophin (hCG). Most genes showed higher expression during the breeding season in both sexes. However, activation of immune responses was much stronger in female goldfish. Injection with hCG, an analog of luteinizing hormone (LH), which is involved in numerous reproductive functions, markedly changed gene expression in most studied organs, in both male and female goldfish. Again, female goldfish were found to be more responsive than male goldfish. The strongest activation of these genes was seen 7 days post-injection; the effect was dose dependent with a lower dose being in general more effective. For several of the genes, the gills were the most responsive tissue and, in male goldfish, gills were often the only responsive tissue, suggesting an important immunological role for gills during breeding. The data suggest that increasing expression levels are regulated by LH arising during the breeding season, with greater sensitivity in female goldfish than in male goldfish. These data support an interaction between the innate immune system and the reproductive axis.

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