您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

De novo transcriptome analysis-gained insights into physiological and metabolic characteristics of Sargassum thunbergii (Fucales, Phaeophyceae)

文献类型： 外文期刊

作者： Liu, Fuli 1 ; Sun, Xiutao 1 ; Wang, Wenjun 1 ; Liang, Zhourui 1 ; Wang, Feijiu 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

期刊名称：JOURNAL OF APPLIED PHYCOLOGY （ 影响因子：3.215； 五年影响因子：3.612 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Sargassum thunbergii has been studied extensively because of its particular ecology and economic value. Unfortunately, further progress has been hampered by lack of available genomic information. In this study, we sequenced and de novo assembled the transcriptome of S. thunbergii. A total of 53,490,957 clean reads were produced and were assembled into 46,269 contigs with the length from 351 nt to 21,538 nt and an average of 1,130 nt. Open reading frame (ORF) prediction in the assembled sequences showed that 30,139 (65.1 %) contigs had ORFs, and BLAST analysis indicated that 28,667 (61.96 %) sequences were annotated with putative functions. A total of 15,166 (32.8 %) putative genes were assigned at least one gene ontology term and 4,263 (10.0 %) putative proteins were classified into 25 categories by aligning against COG/KOG database. Function annotation with KEGG database assigned 11,798 (25.5 %) putative genes to 299 pathways. Out of the six enzymes that may be involved in alginate biosynthesis pathway, four have been identified from the transcriptome. Two pathways of guanosine diphosphate-mannose synthesis were identified in this alga, and the genes encoding the three putative enzymes responsible for mannitol cycle were discovered. Moreover, about 209 putative genes encoding the different kinds of heat shock proteins and a total of 119 putative genes encoding kinds of reactive oxygen species scavengers were also identified in the transcriptome, indicating that S. thunbergii may have strong resistance or tolerance to various stresses. In short, to our knowledge, this is the first data set for S. thunbergii transcriptome and will be a valuable resource for further studies on this alga.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台