您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular characteristics of the HSP70 gene and its differential expression in female and male golden apple snails (Pomacea canaliculata) under temperature stimulation

文献类型： 外文期刊

作者： Song, Hong-Mei 1 ; Mu, Xi-Dong 1 ; Gu, Dang-En 1 ; Luo, Du 1 ; Yang, Ye-Xin 1 ; Xu, Meng 1 ; Luo, Jian-Ren 2 ; Zhang, Jia 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Pe

关键词： Gene expression;HSP70;Pomacea canaliculata;Temperature stress

期刊名称：CELL STRESS & CHAPERONES （ 影响因子：3.667； 五年影响因子：3.717 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Heat-shock protein 70 (HSP70) is one of the most important heat-shock proteins that helps organisms to modulate stress response via over-expression. The HSP70 gene from Pomacea canaliculata was cloned using the RACE approach; the gene is 2,767 bp in length and contains an open reading frame of 1,932 bp, which is encoded by a polypeptide of 643 amino acids. BLAST analysis showed that the predicted amino acid sequence of the P. canaliculata HSP70 gene shared a relatively high similarity with that of other known eukaryotic species that display conserved HSP characteristics. The phylogeny demonstrated a separate clustering of the apple snail HSP70 with other constitutive members from other mollusk species. Quantitative real-time RT-PCR was used to detect the differential expression of HSP70 in both sexes of P. canaliculata at different temperature conditions. These results showed that HSP70 transcript levels decreased slightly under cold shock and increased significantly under heat-shock conditions in both sexes compared to normal temperatures (26°C). Under cold-shock treatment, the sex effect was not significant. With heat treatment, HSP70 expression could be induced at 36°C in both females and males, and it peaked at 42 and 39°C in females and males, respectively. In addition, a clear time-dependent HSP70 expression pattern of the apple snail exposed to the same high temperature (36°C) was observed at different time points. The maximal induction of HSP70 expression appeared at 12 and 48 h in males and females after heat shock, respectively. The maximal induction in females was significantly higher compared to males under heat stimulus. Taken together, these results strongly suggested that males were more susceptible to heat than females and provided useful molecular information for the ecological adaptability of P. canaliculata against extreme environmental stress.

  • 相关文献

[1]Transcriptome analysis between invasive Pomacea canaliculata and indigenous Cipangopaludina cahayensis reveals genomic divergence and diagnostic microsatellite/SSR markers. Mu, Xidong,Song, Hongmei,Luo, Du,Gu, Dangen,Xu, Meng,Luo, Jianren,Hu, Yinchan,Hou, Guangyuan,Xu, Peng,Zhang, Jiaen. 2015

[2]Spatial variation in adult sex ratio across multiple scales in the invasive golden apple snail, Pomacea canaliculata. Xu, Meng,Fang, Miao,Yang, Yexin,Song, Hongmei,Luo, Du,Mu, Xidong,Gu, Dangen,Luo, Jianren,Hu, Yinchang,Fang, Miao,Dick, Jaimie T. A.. 2016

[3]Genetic variability of the invasive snail Pomacea canaliculata in South China based on mitochondrial 16S rDNA sequences. Hu, Yinchang,Mu, Xidong,Luo, Du,Xu, Meng,Yang, Yexin,Gu, Dangen,Luo, Jianren,Zhang, Jiaen.

[4]Molecular identification of Pomacea canaliculata and P. insularum from rice paddy in different origins in China using mitochondrial adenosine triphosphate subunit 6 gene. Bian, Qing-Qing,Li, Xiao-Yan,Fang, Yan-Qin,Jia, Yan-Qing,Mu, Xi-Dong.

[5]Effects of anthraquinone extract from Rheum officinale Bail on the growth performance and physiological responses of Macrobrachium rosenbergii under high temperature stress. Liu, Bo,Xie, Jun,Ge, Xianping,Xu, Pao,Wang, Aiming,Liu, Bo,Xie, Jun,Ge, Xianping,Xu, Pao,He, Yijin,Zhou, Qunlan,Pan, Liangkun,Chen, Ruli,Wang, Aiming.

[6]Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L. An, Meiling,Zheng, Zhou,Miao, Jinlai,Mou, Shanli,Zhang, Xiaowen,Ye, Naihao,Cao, Shaona,Xu, Dong,Fan, Xiao,Wang, Yitao. 2013

[7]半定量RT-PCR方法检测热应激对鲫鱼肝脏中HSP70 mRNA含量的影响. 苏岭,李绍戊,王荻,刘红柏,卢彤岩,尹家胜. 2010

[8]高温刺激导致虾夷扇贝死亡因素的探究. 刘超,吴富村,林思恒,阙华勇,张国范. 2016

[9]菲在厚壳贻贝体内的富集与释放及其对HSP70 mRNA表达的影响. 曾梦妮,李磊,马丽艳,王翠华,张璇,黄冬梅,蒋玫. 2021

[10]饲料蛋白水平对低温应激下吉富罗非鱼血清生化指标和HSP70 mRNA表达的影响. 强俊,杨弘,王辉,徐跑,何杰. 2013

[11]甘露寡糖对异育银鲫生长性能、免疫及HSP70基因表达的影响. 徐磊,刘波,谢骏,戈贤平,张媛媛,崔红红,周群兰,陈汝丽,怀明燕. 2012

[12]中草药方剂对鲫鱼热应激蛋白HSP 70表达的影响. 李绍戊,王荻,苏岭,刘红柏. 2012

[13]黄条(鱼师)hsp70基因克隆及其在早期生长发育过程中的表达调控特性. 方璐,徐永江,崔爱君,姜燕,王滨,周鹤庭,柳学周. 2023

[14]低温应激对吉富罗非鱼血清生化指标及肝脏HSP70基因表达的影响. 刘波,王美垚,谢骏,徐跑,戈贤平,何义进,缪凌鸿,潘良坤. 2011

[15]干露胁迫对脊尾白虾(Exopalaemon carinicauda)HSP70和ferritin基因表达的影响. 王琦,李健,李吉涛,潘鲁青,杨爱国. 2013

[16]氨氮对大口黑鲈北方亚种引进种F1代幼鱼急性毒性及生理变化的影响. 唐忠林,张佳佳,周国勤,强俊,徐跑,徐钢春,王佩佩,庆辉. 2023

[17]急性操作胁迫对美洲鲥亲鱼血清生化指标及HSP70基因表达的影响. 张勇,徐钢春,杜富宽,顾若波. 2016

[18]疏浚泥悬浮液和浸出液对三疣梭子蟹大眼幼体的急性毒性及热应激蛋白70基因表达的影响. 蒋玫,李磊,沈新强,吴庆元,牛俊翔,许高鹏. 2015

[19]溶藻弧菌感染对剑尾鱼HSP70基因表达的影响. 万文菊,王纪亭,石存斌,吴淑勤. 2007

[20]苯并[a]芘(BaP)暴露对三疣梭子蟹(Portunus trituberculatus)P4501A1、P-gp、HSP70基因表达的影响. 许高鹏,蒋玫,李磊,袁骐,吴庆元,牛俊翔,沈新强. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台