中国水产科学研究院机构知识库

Molecular cloning of type I collagen cDNA and nutritional regulation of type I collagen mRNA expression in grass carp

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文献类型：外文期刊

作者： Yu, E. M. ¹ ; Liu, B. H. ¹ ; Wang, G. J. ¹ ; Yu, D. G. ¹ ; Xie, J. ¹ ; Xia, Y. ¹ ; Gong, W. B. ² ; Wang, H. H.; Li, Z. F.;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resource Applicat, Pearl River Fisheries Res Inst, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Ke

关键词： Ctenopharyngodon idella;faba bean;crisp grass carp;muscle hardness

期刊名称：JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION （影响因子：2.13；五年影响因子：2.322 ）

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收录情况： SCI

摘要： Grass carp (Ctenopharyngodon idellus) are important Chinese freshwater fish, and in China, the faba bean has been used as the sole food source for grass carp to transform them into crisp grass carp. Because of this, crisp grass carp has become an economically important fish because of its increased muscle hardness. To study the nutritional regulation of type I collagen in faba bean-fed grass carp, we isolated type I collagen alpha 2 (COL1A2) on the basis of our isolation of COL1A1. The COL1A2 cDNA was found to be 4899 bp in length and included a 4059-bp coding sequence (CDS) and encoded a polypeptide of 1352 AA. The protein peptide molecular weight was 127.39 kD, and the theoretical isoelectric point was 9.37. The COL1A2 protein possessed five alpha-helixes, eight beta-sheets, 16 regions of triple helical repeats, 21 low-complexity regions, 10 function domains and two zinc-binding sites; however, no calcium-binding sites were observed. The mRNA expression of COL1A1 and COL1A2 was assessed in eight tissues (muscle, hepatopancreas, intestine, gills, skin, fin, kidney and spleen) from grass carp and crisp grass carp by semi-quantitative RT-PCR. Expression of COL1A1 in the muscle, intestines and skin of crisp grass carp was higher than that in grass carp, and expression of COL1A2 in the muscle, gills, fin and skin of crisp grass carp was higher than that in grass carp. In the muscle of crisp grass carp, expression of COL1A1 and COL1A2 was higher than that in grass carp, which was further confirmed by real-time PCR, and collagen content also was enhanced. These results demonstrated that type I collagen was closely related to the increased muscle hardness of faba bean-fed grass carp

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意见箱

Molecular cloning of type I collagen cDNA and nutritional regulation of type I collagen mRNA expression in grass carp

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意 见 箱

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意见箱