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Large-scale preparation and multi-dimensional characterization of high-purity mycotoxin deoxynivalenol in rice culture inoculated with Fusarium graminearum

文献类型： 外文期刊

作者： Zhao, Zhiyong 1 ; Song, Suquan 1 ; Liu, Na 1 ; Rao, Qinxiong 1 ; Hou, Jiafa 2 ; De Saeger, Sarah 3 ; Wu, Aibo 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Lab Qual & Safety Risk Assessment Agroprod Shangh, Minist Agr, Shanghai 201403, Peoples R China

2.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

3.Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium

期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

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收录情况： SCI

摘要： Food safety monitoring and toxicological research of mycotoxins are still in need of large quantities of high-purity deoxynivalenol (DON). To attain this purpose, a rapid, economical and reproducible purification method was developed for large-scale production of DON from rice culture inoculated with a DON-producing Fusarium graminearum strain JYH. The inoculated rice culture was first extracted with acetonitrile-water (84/16, v/v). The extracts were evaporated to dryness on a rotary evaporator after ethyl acetate partitioning and then dissolved in water followed by the final purification procedure through preparative high performance liquid chromatography and montmorillonite treatment. A combined approach of ultraviolet spectrometry (UV), ultra high performance liquid chromatography (UHPLC), mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy was applied for the multi-dimensional characterization of the target compound. As a result, the recovery of DON from the crude extract to the final product was up to 70%. An amount of 150 mg DON with the desirable purity of 98.93% could be obtained from 100 g of rice culture, which possessed identical immunochemical characteristics compared to a certified commercial DON standard. This proposed strategy might act as a valuable reference to obtain rather expensive compounds in a straightforward way.

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