Differentiation in MALDI-TOF MS and FTIR spectra between two pathovars of Xanthomonas oryzae
文献类型： 外文期刊
作者： Ge, Mengyu 1 ; Li, Bin 1 ; Wang, Li 1 ; Tao, Zhongyun 1 ; Mao, Shengfeng 2 ; Wang, Yangli 3 ; Xie, Guanlin 1 ; Sun, Guoch 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China
2.Zhejiang Agr & Forestry Univ, State Key Lab Cultivat Base Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021,
关键词： Xanthomonas oryzae pv. oryzae;Xanthomonas oryzae pv. oryzicola;Pathovars;MALDI-TOF MS;FTIR spectra;Distinction
期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）
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收录情况： SCI
摘要： Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) strains are closely related phenotypically and genetically, which make it difficult to differentiate between the two pathovars based on phenotypic and DNA-based methods. In this study, a fast and accurate method was developed based on the differences in MALDI-TOF MS and FTIR spectra between the two pathovars. MALDI-TOF MS analysis revealed that 9 and 10 peaks are specific to Xoo and Xoc, respectively, which can be used as biomarkers to identify and differentiate the two closely related pathovars. Furthermore, FTIR analysis showed that there is a significant difference in both the band frequencies and absorption intensity of various functional groups between the two pathovars. In particular, the 6 peaks at 3433, 2867, 1273, 1065, 983 and 951 cm~(-1) were specific to the Xoo strains, while one peak at 1572 cm~(-1) was specific to the Xoc strains. Overall, this study gives the first attempt to identify and differentiate the two pathovars of X. oryzae based on mass and FTIR spectra, which will be helpful for the early detection and prevention of the two rice diseases caused by both X. oryzae pathovars.
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