Determination of biogenic amines in traditional Chinese fermented foods by reversed-phase high-performance liquid chromatography (RP-HPLC)
文献类型: 外文期刊
作者: Gong, Xiao 1 ; Wang, Xiaoxi 2 ; Qi, Ningli 3 ; Li, Jihua 1 ; Lin, Lijing 1 ; Han, Zhiping 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Key Lab Trop Crop Prod Proc, Chinese Agr Minist, Zhanjiang, Peoples R China
2.Wageningen Univ, NL-6700 AP Wageningen, Netherlands
3.Minist Agr, Ctr Food Qual Supervis & Testing, Zhanjiang, Peoples R China
关键词: biogenic amines;fermented foods;household production;HPLC analysis;diode array detector
期刊名称:FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT ( 影响因子:3.057; 五年影响因子:2.96 )
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收录情况: SCI
摘要: A survey of biogenic amine content of traditional Chinese fermented foods (douchi, sufu, fermented sausage, yulu, and shrimp paste) was carried out. Eight major biogenic amines including putrescine, cadaverine, histamine, phenylethylamine, tyramine, spermine, spermidine and tryptamine were separated by reversed-phase HPLC-DAD on Inertsil ODS-SP column after pre-column derivatisation with dansyl chloride. The results showed that spermine (1.65-3.96 mg 100 g(-1)), putrescine (0.20-10.89 mg 100 g(-1)), cadaverine (3.60-12.14 mg 100 g(-1)), and histamine (0.57-20.24 mg 100 g(-1)) were the most represented amines. All amines were detected in yulu, while sufu showed a much higher content of most amines. Shrimp paste showed the lowest level of total biogenic amines. Moreover, the composition and content of eight biogenic amines in the selected samples varied among different food types, origins and batches. Although the average content for each amine is within the range that may elicit direct adverse reactions, consumers should be aware of the potential synergistic effect among different amines and limit their consumption at each meal
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