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Pyramiding expression of maize genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic wheat

文献类型： 外文期刊

作者： Zhang, HuiFang 1 ; Xu, WeiGang 2 ; Wang, HuiWei 2 ; Hu, Lin 2 ; Li, Yan 2 ; Qi, XueLi 2 ; Zhang, Lei 2 ; Li, ChunXin 2 ; Hua 1 ;

作者机构： 1.Henan Agr Univ, Coll Agron, Zhengzhou 450002, Henan, Peoples R China

2.Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou 450002, Henan, Peoples R China

关键词： Phosphoenolpyruvate carboxylase (PEPC);Pyruvate orthophosphate dikinase (PPDK);Transgenic wheat;Gene expression;Photosynthesis characters

期刊名称：PROTOPLASMA （ 影响因子：3.356； 五年影响因子：3.298 ）

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收录情况： SCI

摘要： Using particle bombardment transformation, we introduced maize pepc cDNA encoding phosphoenolpyruvate carboxylase (PEPC) and ppdk cDNA encoding pyruvate orthophosphate dikinase (PPDK) into the C_3 crop wheat to generate transgenic wheat lines carrying cDNA of pepc (PC lines), ppdk (PK lines) or both (PKC lines). The integration, transcription, and expression of the foreign genes were confirmed by Southern blot, Real-time quantitative reverse transcription PCR (Q-RT–PCR), and Western blot analysis. Q-RT-PCR results indicated that the average relative expression levels of pepc and ppdk in the PKC lines reached 10 and 4.6, respectively, compared to their expressions in untransformed plants (set to 1). The enzyme activities of PEPC and PPDK in the PKC lines were 4.3- and 2.1-fold higher, respectively, than in the untransformed control. The maximum daily net photosynthetic rates of the PKC, PC, and PK lines were enhanced by 26.4, 13.3, and 4.5 %, respectively, whereas the diurnal accumulations of photosynthesis were 21.3, 13.9, and 6.9 %, respectively, higher than in the control. The F_v/F_m of the transgenic plants decreased less than in the control under high temperature and high light conditions (2 weeks after anthesis), suggesting that the transgenic wheat transports more absorbed light energy into a photochemical reaction. The exogenous maize C_4-specific pepc gene was more effective than ppdk at improving the photosynthetic performance and yield characteristics of transgenic wheat, while the two genes showed a synergistic effect when they were transformed into the same genetic background, because the PKC lines exhibited improved photosynthetic and physiological traits.

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