A New Method for Determination of alpha-Tocopherol in Tropical Fruits by Ultra Performance Convergence Chromatography with Diode Array Detector
文献类型: 外文期刊
作者: Gong, Xiao 1 ; Qi, Ningli 1 ; Wang, Xiaoxi 2 ; Li, Jihua 1 ; Lin, Lijing 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Minist Agr, Key Lab Trop Crop Prod Proc, Zhanjiang 524001, Peoples R China
2.Wageningen Univ, NL-6708 PB Wageningen, Netherlands
关键词: alpha-Tocopherol;Tropical fruits;Ultra performance;convergence;chromatography (UPC2);Diode array detector
期刊名称:FOOD ANALYTICAL METHODS ( 影响因子:3.366; 五年影响因子:3.07 )
ISSN:
年卷期:
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收录情况: SCI
摘要: A new method for the analysis of alpha-tocopherol in tropical fruits by ultra performance convergence chromatography (UPC2) was developed for the first time. Five varieties of tropical fruit samples were separately saponified under classical heating and extracted with ether. The extracted alpha-tocopherol was separated on a BEH column, with a mobile phase consisting of CO2 and methanol, with a gradient elution (99:1 to 90:10), and detected with diode array detector at 293 nm. The limit of detection (LOD) and limit of quantification (LOQ) were about 60.0 and 103.3 ng, respectively. This method was considered to be simple, fast and reliable, and successfully applied to analysis of alpha-tocopherol in tropical fruits. The values of alpha-tocopherol in pitaya, jackfruit, durians, mango, and papaya ranged from 0.16 to 0.45 mg/100 g dry weight in edible portion. Recovery rates obtained by the standard addition method on these tropical fruit samples ranged from 95.4 to 101.4 % with high repeatability (RSD, 1.2-2.6 %).
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