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Development of a rapid cyprinid herpesvirus 2 detection method by loop-mediated isothermal amplification

文献类型: 外文期刊

作者: Liang, L. -G. 1 ; Xie, J. 1 ; Luo, D. 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R China

2.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou, Peoples R China

关键词: Carassius auratus gibelio;cyprinid herpesvirus 2;loop-mediated isothermal amplification;PCR;rapid detection

期刊名称:LETTERS IN APPLIED MICROBIOLOGY ( 影响因子:2.858; 五年影响因子:2.776 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Significance and Impact of the Study: Herpesviral haematopoietic necrosis, caused by cyprinid herpesvirus 2 (CyHV-2), is a severe disease of goldfish and Prussian carp associated with high mortality. We developed a loop-mediated isothermal amplification (LAMP) assay to detect CyHV-2 at relatively low plasmid DNA copy levels. The results show that the LAMP assay has a number of advantages (simple, sensitive, rapid and specific) over the conventional polymerase chain reaction and can be applied in the laboratory and field. Particularly, the method is highly applicable to facilitate surveillance and early diagnosis of CyHV-2

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