Transcription factor MdCBF1 gene increases freezing stress tolerance in transgenic Arabidopsis thaliana
文献类型： 外文期刊
作者： Xue, Y. 1 ; Wang, Y. Y. 1 ; Peng, R. H. 1 ; Zhen, J. L. 1 ; Zhu, B. 1 ; Gao, J. J. 1 ; Zhao, W. 1 ; Han, H. J. 1 ; Yao, Q. H 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotech Res Inst, Shanghai Key Lab Agr Genet Breeding, Shanghai 201106, Peoples R China
关键词： amino acid sequencem applem dehydration responsive element;Malus domestica;RT-PCR
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）
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收录情况： SCI
摘要： Transcription factors play vital roles in stress signal transduction and gene expression modulation. The sequence analysis shows that MdCBF1 from Malus domestica Borkh. cv. Fuji contained an AP2 core domain of 56 amino acids. By comparison of deduced amino acid sequences of CBF related proteins, we deduced that MdCBF1 is a CBF transcription factor gene which belongs to AP2/EREBP family, DREB-A1 subfamily. Further, we reported that transgenic Arabidopsis thaliana plants expressing the MdCBF1 gene exhibited stronger growth than wild type plants under freezing stress. The analysis of RT-PCR for stress-responsive genes implied that MdCBF1 over-expressing plants had a higher expression of COR15a, RD29A, and RD29B genes than wild type plants. Collectively, our results indicate that MdCBF1 might play an important role in the response of transgenic Arabidopsis plants to freezing stress
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