Transcriptome comparison between inactivated and activated ovaries of the honey bee Apis mellifera L.
文献类型： 外文期刊
作者： Niu, D. 1 ; Zheng, H. 1 ; Corona, M. 2 ; Lu, Y. 1 ; Chen, X. 3 ; Cao, L. 4 ; Sohr, A. 2 ; Hu, F. 1 ;
作者机构： 1.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.USDA ARS, Bee Res Lab, Beltsville, MD USA
3.Zhejiang Acad TCM, Inst Basic Med, Hangzhou, Zhejiang, Peoples R China
4.Zhejiang Acad Agr Sci, Anim Husb & Vet Res Inst, Hangzhou, Zhejiang, Peoples R China
关键词： Apis mellifera;inactivated ovaries;activated ovaries;RNA-sequencing;differentially expressed genes;mRNAs
期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Ovarian activity not only influences fertility, but is also involved with the regulation of division of labour between reproductive and behavioural castes of female honey bees. In order to identify candidate genes associated with ovarian activity, we compared the gene expression patterns between inactivated and activated ovaries of queens and workers by means of high-throughput RNA-sequencing technology. A total of 1615 differentially expressed genes (DEGs) was detected between ovaries of virgin and mated queens, and more than 5300 DEGs were detected between inactivated and activated worker ovaries. Intersection analysis of DEGs amongst five libraries revealed that a similar set of genes (824) participated in the ovary activation of both queens and workers. A large number of these DEGs were predominantly related to cellular, cell and cell part, binding, biological regulation and metabolic processes. In addition, over 1000 DEGs were linked to more than 230 components of Kyoto Encyclopedia of Genes and Genomes pathways, including 25 signalling pathways. The reliability of the RNA-sequencing results was confirmed by means of quantitative real-time PCR. Our results provide new insights into the molecular mechanisms involved in ovary activation and reproductive division of labour.
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