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Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic Fusarium oxysporum CS-20

文献类型： 外文期刊

作者： Pu, Xiaoming 2 ; Xie, Bingyan 3 ; Li, Peiqian 1 ; Mao, Zhenchuan 4 ; Ling, Jian; Shen, Huifang 2 ; Zhang, Jingxin 2 ;

作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

2.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab New Tech Plant Protect Guangdong, Guangzhou 510640, Guangdong, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100193, Peoples R China

4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beiji

关键词： indirect antagonism;hydroponic chamber;ISR;SAR.

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

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收录情况： SCI

摘要： A defence response can be induced by nonpathogenic Fusarium oxysporum CS-20 in several crops, but the molecular mechanism has not been clearly demonstrated. In the present study, we analysed the defence mechanism of a susceptible cucumber cultivar (Cucumis sativus L. 9930) against a pathogen (F. oxysporum f. sp. cucumerinum) through the root precolonization of CS-20. A challenge inoculation assay indicated that the disease severity index (DSI) was reduced, ranging from 18.83 to 61.67 in comparison with the pathogen control. Root colonization analysis indicated that CS-20 clearly did not appear to influence the growth of cucumber seedlings. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) revealed that CS-20-mediated defence response was activated by PR3, LOX1 and PAL1 and the pathogenmediated resistance response was regulated by PR1 and PR3. Moreover, both nonpathogenic and pathogenic F. oxysporum were able to upregulate NPR1 expression. In contrast to a pathogen, CS-20 can activate the Ca2+/CaM signal transduction pathway, and the gene expression of both CsCam7 and CsCam12 increased significantly. The gene expression analysis indicated that CS-20 strongly enhanced the expression of PR3, LOX1, PAL1, NPR1, CsCam7 and CsCam12 after inoculation. Overall, the defence response induced by CS-20 can be controlled by multiple genes in the cucumber plant.

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