A rabbit monoclonal antibody-based sensitive competitive indirect enzyme-linked immunoassay for rapid detection of chloramphenicol residue
文献类型： 外文期刊
作者： Liu, Na 1 ; Song, Suquan 2 ; Lu, Lei 1 ; Nie, Dongxia 2 ; Han, Zheng 2 ; Yang, Xianli 2 ; Zhao, Zhihui; Wu, Aibo; Zhen 1 ;
作者机构： 1.Zhejiang Univ, Sch Biosyst Engn & Food Sci, Hangzhou 310003, Zhejiang, Peoples R China
2.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Lab Qual & Safety Risk Assessment Agroprod Shangh, Minist Agr, Shanghai, Peoples R China
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： A sensitive competitive indirect enzyme-linked immunoassay (ciELISA) based on a rabbit monoclonal antibody (RabMAb) against chloramphenicol (CAP) has been developed and validated in this study. After optimisation of several key physico-chemical factors, such as Tween-20 percentage, pH value and ionic strength, the immunoassay showed excellent performance within the linear range of 0.18-6.37 ng mL(-1), with the 50% inhibition concentration (IC50) of 1.06 ng mL(-1) and the limit of detection (LOD) of 0.1 ng mL(-1). In addition, the cross-reactivities of RabMAb towards chloramphenicol succinate, florfenicol and thiamphenicol were 2.09, 12.45 and 18.10%, respectively. Finally, the developed method was applied in spiked swine urine, milk and honey samples, with recoveries ranging from 71.03 to 109.62%. The result demonstrated that the developed immunoassay is a valuable method for screening and quantitation of CAP residues in real samples
- 相关文献
作者其他论文 更多>>
-
2D/2D Co3O4/BiOCl nanocomposite with enhanced antibacterial activity under full spectrum: Synergism of mesoporous structure, photothermal effect and photocatalytic reactive oxygen species
作者：Wang, Xuesheng;Zhang, Zhijie;Shao, Yi;Yao, Chunxia;Huang, Liujuan;Song, Wei;Yang, Xianli
关键词：BiOCl; Photothermal effect; Antibacterial; Photocatalysis
-
Characterization and phylogenetic analysis of the complete mitochondrial genome sequence of Lagenaria siceraria, a cucurbit crop
作者：Du, Xuan;Wang, Kuanhong;Yang, Xiaofeng;Zhang, Hongmei;Liu, Na;Zhang, Zhaohui;Tang, Yuying;Wu, Jue
关键词：Lagenaria siceraria; mitochondrial genome; phylogenetic analysis; cucurbit; evolutionary analysis
-
Enhanced Electrochemical and Photochemical Sensors for In Vivo Biological Monitoring Based on Carbon Nanomaterials
作者：Huang, Qingwen;Lin, Xialin;Nie, Dongxia;Wang, Jie;Fan, Kai;Zhao, Zhihui;Han, Zheng;Lin, Xialin;Zhao, Zhihui
关键词：carbon nanomaterials; electrochemical sensors; in vivo detection; photochemical sensor
-
Inhibition Effects of Infrared Radiation Prior to Cold Storage Against Alternaria alternata on Yellow Peach (Amygdalus persica)
作者：Liu, Longxiao;Li, Zhizhong;Liu, Longxiao;Fan, Kai;Huang, Qingwen;Wang, Xinyi;Nie, Dongxia;Han, Zheng;Zhao, Zhihui
关键词：
Alternaria alternata ;Alternaria toxins; infrared radiation; yellow peach; inhibitory effect -
A host-guest recognition electrochemical sensor based on MXene/COF heterocomposite bound to β-cyclodextrin for the direct detection of zearalenone in cereals
作者：Fei, Qianqian;Bian, Ming;Han, Zheng;Fei, Qianqian;Huang, Qingwen;Nie, Dongxia;Fan, Kai;Wang, Xinyi;Zhao, Zhihui;Han, Zheng
关键词：Host-guest recognition; Label-free electrochemical sensors; Zearalenone; beta-Cyclodextrin; Covalent organic framework
-
Magnetic beads-based double-stranded DNA fluorescent aptasensor biosensor for deoxynivalenol detection
作者：Meng, Jiajia;Li, Ruijiao;Chen, Xinyue;Wang, Xinyi;Huang, Qingwen;Nie, Dongxia;Guo, Dakai;Zhao, Zhihui;Han, Zheng
关键词：Fluorescent aptasensor; Double-stranded DNA; Deoxynivalenol; Magnetic beans; Aptamer
-
Molecularly Imprinted Electrochemical Sensor Based on Au-MoS2/MOF(Fe2+/Fe3+) for Rapid Detection of Tenuazonic Acid
作者：Liu, Ming-Hui;Nie, Dong-Xia;Liu, Ming-Hui;Cheng, Meng;Guo, Da-Kai;Han, Zheng;Nie, Dong-Xia
关键词：Molecular imprinting; Au-MoS2/MOF(Fe2+/Fe3+); Electrochemical sensor; Tenuazonic acid; Catalytic cycle amplification
