A rabbit monoclonal antibody-based sensitive competitive indirect enzyme-linked immunoassay for rapid detection of chloramphenicol residue
文献类型： 外文期刊
作者： Liu, Na 1 ; Song, Suquan 2 ; Lu, Lei 1 ; Nie, Dongxia 2 ; Han, Zheng 2 ; Yang, Xianli 2 ; Zhao, Zhihui; Wu, Aibo; Zhen 1 ;
作者机构： 1.Zhejiang Univ, Sch Biosyst Engn & Food Sci, Hangzhou 310003, Zhejiang, Peoples R China
2.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Lab Qual & Safety Risk Assessment Agroprod Shangh, Minist Agr, Shanghai, Peoples R China
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
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收录情况： SCI
摘要： A sensitive competitive indirect enzyme-linked immunoassay (ciELISA) based on a rabbit monoclonal antibody (RabMAb) against chloramphenicol (CAP) has been developed and validated in this study. After optimisation of several key physico-chemical factors, such as Tween-20 percentage, pH value and ionic strength, the immunoassay showed excellent performance within the linear range of 0.18-6.37 ng mL(-1), with the 50% inhibition concentration (IC50) of 1.06 ng mL(-1) and the limit of detection (LOD) of 0.1 ng mL(-1). In addition, the cross-reactivities of RabMAb towards chloramphenicol succinate, florfenicol and thiamphenicol were 2.09, 12.45 and 18.10%, respectively. Finally, the developed method was applied in spiked swine urine, milk and honey samples, with recoveries ranging from 71.03 to 109.62%. The result demonstrated that the developed immunoassay is a valuable method for screening and quantitation of CAP residues in real samples
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