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Cysteine Protease 51 (CP51), an anther-specific cysteine protease gene, is essential for pollen exine formation in Arabidopsis

文献类型： 外文期刊

作者： Yang, Yongxue 1 ; Dong, Caihua 1 ; Yu, Jingyin 1 ; Shi, Lei 3 ; Tong, Chaobo 1 ; Li, Zhenbo 4 ; Huang, Junyan 1 ; Liu, Sh 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Oil Crops Res Inst, Wuhan 430345, Peoples R China

2.Wuhan Acad Agr Sci & Technol, Wuhan Inst Agr Sci, Wuhan 430345, Peoples R China

3.Henan Acad Agr Sci, Key Lab Oil Crops Huanghuaihai Plains, Henan Prov Key Lab Oil Crops Improvement, Minist Agr,Ind Crops Res Inst, Zhengzhou 450002, Peoples R China

4.So

关键词： Cysteine proteases;CP51;Pollen exine;Male sterility;Arabidopsis

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 2020影响因子：2.711； 五年影响因子：2.73 ）

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收录情况： SCI

摘要： Cysteine proteases play important roles in intracellular protein degradation, programmed cell death and responses to environmental stimuli in plant. Although subclassification and biochemical analysis of major plant papain-like cysteine proteases (PLCPs) have been studied, the biological function of many PLCPs remained unknown. In this study, we identified a PLCP gene Cysteine Protease 51 (CP51) which participates in exine formation and anther development in Arabidopsis thaliana. Promoter-GUS fusion detection showed its specific expression in anthers at stages 9-12. RNA interference (RNAi) transgenic plants with reduced CP51 transcriptional levels exhibited a male sterile phenotype with aborted microspores, shortened siliques and fewer or no seeds. Cytological analysis indicated that pollen abortion occurred due to defective pollen exine and the tapetum degraded earlier during the transition from the uninucleated stage to the binucleated stage. Scanning electron microscopy demonstrated that aborted microspores lacked complete or normal reticulate exine, and the intine membrane was extruded in the pollens of CP51-RNAi plants. Transmission electron microscopy further revealed that the tapetum degeneration was initiated early and that normal tectum connections to the bacula were missing in anthers of CP51-RNAi plants. Taken together, these results suggested that CP51 critically mediates tapetum stability and pollen exine formation.

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