Identification of promoter polymorphisms in the cytochrome P450 CYP6AY1 linked with insecticide resistance in the brown planthopper, Nilaparvata lugens
文献类型： 外文期刊
作者： Pang, R. 1 ; Li, Y. 2 ; Dong, Y. 1 ; Liang, Z. 1 ; Zhang, Y. 2 ; Zhang, W. 1 ;
作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Key Lab Biodivers Dynam & Conservat, Guangdong Higher Educ Inst,State Key Lab Biocontr, Guangzhou 510275, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Inst Plant Protect, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou, Guangdong, Peoples R China
关键词： P450;promoter polymorphisms;imidacloprid;buprofezin
期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）
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收录情况： SCI
摘要： Imidacloprid resistance in the brown planthopper, Nilaparvata lugens, is primarily the result of the over-expression of cytochrome P450 monooxygenases. Here, a field-collected strain of N.lugens was shown to be highly resistant to both imidacloprid and buprofezin. Insecticide exposure and quantitative real-time PCR revealed that its resistance was mainly associated with a cytochrome P450 gene, CYP6AY1. CYP6AY1 is known to metabolize imidacloprid but its effect on buprofezin is unclear. In the 5-untranslated region of CYP6AY1, a novel alternative splicing was detected. After a 1990-bp promoter region was cloned, its basal luciferase activity was assessed. Furthermore, genotyping studies identified 12 variations in the promoter region that discriminated between the field-collected and control strain. Finally, survival bioassays revealed a single nucleotide polymorphism and an insertion-deletion polymorphism linked to buprofezin and imidacloprid resistance. Mutagenesis of these sites enhanced the promoter activity of CYP6AY1. These results suggest that promoter polymorphisms may affect P450-mediated multiple insecticide resistance of pests.
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