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Monitoring the progression of loop-mediated isothermal amplification using conductivity

文献类型： 外文期刊

作者： Zhang, Xuzhi 1 ; Liu, Wenwen 2 ; Lu, Xun 3 ; Gooding, J. Justin 3 ; Li, Qiufen 1 ; Qu, Keming 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China

3.Univ New S Wales, Sch Chem, Sydney, NSW 2052, Australia

4.Univ New S Wales, Australian Ctr NanoMed, Sydney, NSW 2052, Australia

关键词： Loop-mediated isothermal amplification;Conductivity;Monitor biochemical reaction;Gene analysis method

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Loop-mediated isothermal amplification (LAMP) yields a large amount of DNA, as well as magnesium pyrophosphate precipitate, causing a decrease in ionic strength that can be measured with a conductivity meter. There is a clear relationship between the conductivity of the LAMP mixture solution and the duration of biochemical reaction. Moreover, there is also a clear relationship between the change in conductivity and the amount of initial template DNA over the range of 0.08 to 3.2 ng. These results demonstrate the feasibility not only for detecting the LAMP product qualitatively but also for real-time monitoring the biochemical reaction progression quantitatively using conductivity measurements. (C) 2014 Elsevier Inc. All rights reserved.

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